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ZFIN ID: ZDB-FIG-140417-9
Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biology Open   2(11):1203-1213 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 2

Expression of Hedgehog target genes in wild-type and gli mutant embryos.

ptch2, prdm1a, nkx2.2a and olig2 were examined in WT (A–D), gli1ts269/ts269 (E–H), gli2ai276/i276 (I–L), gli1ts269/ts269;gli2ai276/i276 (M–P), gli2ai276/ty119 (Q–T), and gli2aty119/ty119 (U–X) by WISH. All specimens were fixed at 24hpf, except those hybridized with prdm1a probe (18 somites).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.2a WT standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 neural tube ventral region ISH
gli1ts269/ts269 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 neural tube ventral region ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 Not Detected midbrain ISH
Prim-5 neural tube ventral region ISH
gli2ai276/i276 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 neural tube ventral region ISH
gli2aty119/+; gli2ai276/+ standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 neural tube ventral region ISH
gli2aty119/ty119 standard conditions Prim-5 forebrain ISH
Prim-5 neural tube ventral region ISH
olig2 WT standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
gli1ts269/ts269 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
gli2ai276/i276 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
gli2aty119/+; gli2ai276/+ standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
gli2aty119/ty119 standard conditions Prim-5 forebrain ISH
Prim-5 midbrain ISH
Prim-5 motor neuron ISH
prdm1a WT standard conditions 14-19 somites adaxial cell ISH
gli1ts269/ts269 standard conditions 14-19 somites adaxial cell ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions 14-19 somites Not Detected adaxial cell ISH
gli2ai276/i276 standard conditions 14-19 somites adaxial cell ISH
gli2aty119/+; gli2ai276/+ standard conditions 14-19 somites adaxial cell ISH
gli2aty119/ty119 standard conditions 14-19 somites adaxial cell ISH
ptch2 WT standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
gli1ts269/ts269 standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
gli2ai276/i276 standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
gli2aty119/+; gli2ai276/+ standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
gli2aty119/ty119 standard conditions Prim-5 central nervous system ISH
Prim-5 somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open