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ZIRC
ZFIN ID: ZDB-FIG-140417-10
Wang et al., 2013 - Targeted inactivation and identification of targets of the Gli2a transcription factor in the zebrafish. Biology Open   2(11):1203-1213 Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 3

Gli1 and Gli2a act redundantly to pattern the zebrafish myotome.

(A,E) Wild-type (WT); (B,F) gli2ai276; (C,G) gli1ts269 and (D,H) gli1ts269; gli2ai276 24hpf embryos stained with anti Prox1a (green) and either mAb F59 (red: panels A–D) or 4D9 (red: panels E–H) to reveal slow-twitch muscle and MP fibres. (I–K) Transverse sections of embryos shown in panels E–G. (L–O) Dorsal views of the caudal regions of 18ss embryos hybridized with a probe for myod1 transcript. (L) WT; (M) gli2ai276; (N) gli1ts269 (O) gli1ts269;gli2ai276.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT standard conditions 14-19 somites somite ISH
gli1ts269/ts269 standard conditions 14-19 somites somite ISH
gli1ts269/ts269; gli2ai276/i276 standard conditions 14-19 somites somite ISH
gli2ai276/i276 standard conditions 14-19 somites somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-F59 IHC WT standard conditions Prim-5 slow muscle cell
IHC gli1ts269/ts269 standard conditions Prim-5 slow muscle cell
IHC gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 slow muscle cell
IHC gli2ai276/i276 standard conditions Prim-5 slow muscle cell
Ab1-eng IHC WT standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
IHC gli1ts269/ts269 standard conditions Prim-5 Not Detected fast muscle cell
IHC Prim-5 Not Detected muscle pioneer
IHC gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
IHC gli2ai276/i276 standard conditions Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
gli1ts269/ts269; gli2ai276/i276 standard conditions Prim-5 somite has fewer parts of type slow muscle cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open