ZFIN ID: ZDB-FIG-130722-4
Tseng et al., 2011 - An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish. Developmental Biology   357(2):541-57 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 30%-epiboly

Fig. 3 Genes that are affected after knockdown of otx2 (I), gata5 (II), gata6 (III) and prdm1a (IV) at 5 hpf. (I) otx2 MO represses many genes′ expression at 5 hpf. (II) gata5 MO affect many genes′ expression at 5 hpf. (III) gata6 MO inhibit many genes′ expression at 5 hpf. (IV) prdm1a MO inhibit the expression of cdx4, og9x and foxa2 at 5 hpf. The Q-PCR results from different morphants are shown in bar graphs. The gene expression levels in the control embryos were used as standards and are shown by blue bars. The red bars represent the extent (fold-change) to which gene expression was up- or down-regulated in morphants compared with control embryos. The results were obtained from more than three sample batches, and the standard deviations are indicated as lines extending from the mean. The statistical significance of the quantitative data was determined by the Student′s t-test. ★★, represents p < 0.01, ★, represents 0.01 < p < 0.05. I(B–O), II(B–I), III(B–M), and IV(B–G): Representative images from in situ hybridization show the domains in which the expressed genes were deregulated in morphants. Upper images: expression of the gene (name shown in the right lower corner) in the control MO-injected embryos; lower images: expression of the gene (name shown in the right lower corner) in the otx2 MO (I), gata5 MO (II), gata6 MO (III), and prdm1a MO (IV) injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdx4 WT control 30%-epiboly DEL ISH
WT + MO1-prdm1a standard conditions 30%-epiboly DEL ISH
cers2a WT control 30%-epiboly whole organism ISH
WT + MO3-gata5 standard conditions 30%-epiboly whole organism ISH
foxa2 WT control 30%-epiboly margin ISH
WT + MO1-gata6 standard conditions 30%-epiboly margin ISH
WT + MO1-otx2b standard conditions 30%-epiboly margin ISH
WT + MO1-prdm1a standard conditions 30%-epiboly margin ISH
foxa3 WT control 30%-epiboly whole organism ISH
WT + MO1-gata6 standard conditions 30%-epiboly whole organism ISH
WT + MO1-otx2b standard conditions 30%-epiboly whole organism ISH
gata5 WT control 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
WT + MO1-gata6 standard conditions 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
WT + MO1-otx2b standard conditions 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
gata6 WT control 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
WT + MO1-otx2b standard conditions 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
WT + MO3-gata5 standard conditions 30%-epiboly DEL ISH
30%-epiboly yolk syncytial layer ISH
irx3a WT control 30%-epiboly presumptive endoderm ISH
WT + MO3-gata5 standard conditions 30%-epiboly presumptive endoderm ISH
sebox WT control 30%-epiboly presumptive endoderm ISH
WT + MO1-gata6 standard conditions 30%-epiboly presumptive endoderm ISH
WT + MO1-otx2b standard conditions 30%-epiboly presumptive endoderm ISH
WT + MO1-prdm1a standard conditions 30%-epiboly presumptive endoderm ISH
sox17 WT control 30%-epiboly presumptive endoderm ISH
WT + MO1-otx2b standard conditions 30%-epiboly presumptive endoderm ISH
sox32 WT control 30%-epiboly presumptive endoderm ISH
WT + MO1-gata6 standard conditions 30%-epiboly presumptive endoderm ISH
WT + MO1-otx2b standard conditions 30%-epiboly presumptive endoderm ISH
tbx16 WT control 30%-epiboly presumptive mesoderm ISH
WT + MO1-gata6 standard conditions 30%-epiboly presumptive mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 357(2), Tseng, W.F., Jang, T.H., Huang, C.B., and Yuh, C.H., An evolutionarily conserved kernel of gata5, gata6, otx2 and prdm1a operates in the formation of endoderm in zebrafish, 541-57, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.