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ZFIN ID: ZDB-FIG-121212-30
Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development. PLoS One   7(11):e49357 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 7 The combined knockdown of Otx and Mitf transcription factors creates a more severe phenotype.

(A) The effect of otx1a/otx2 knockdown was compared between wildtype, mitfaw2 and mitfbhu3857 single mutants, and mitfbhu3857;mitfaw2 double mutants. Morpholinos were injected at a total concentration of 2 ng/embryo using the same needle for all genotypes. Results from one representative experiment are shown. The percentage of severe RPE defects was significantly increased (P<0.0001) in the mitfbhu3857;mitfaw2 double mutant background compared to wildtype and single mitfa and mitfb mutants. Wildtype, N = 252; mitfa, N = 196; mitfb, N = 158; mitfa;mitfb, N = 170. (B,C) At 24 hpf dct expression is nearly eliminated in mitfbhu3857;mitfaw2 embryos injected with the otx1a/otx2 morpholino combination (C) compared to sibling embryos injected with control morpholino (B).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dct mitfaw2/w2; mitfbhu3857/hu3857 standard conditions Prim-5 optic cup ISH
mitfaw2/w2; mitfbhu3857/hu3857 + MO1-otx2a + MO1-otx2b standard conditions Prim-5 optic cup ISH
otx2a WT standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2 standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2; mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
otx2b WT standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2 standard conditions 20-25 somites central nervous system ISH
mitfaw2/w2; mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
mitfbhu3857/hu3857 standard conditions 20-25 somites central nervous system ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One