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ZIRC
ZFIN ID: ZDB-FIG-121212-27
Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development. PLoS One   7(11):e49357 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 4 Otx knockdown causes a decrease in dct expression in the developing RPE.

dopachrome tautomerase (dct) mRNA expression was analyzed through in situ hybridization at 24 hpf in otx morphants. dct expression was reduced only in the ventral most region of the optic cup in single morphants (B–D, white arrows) compared to controls (A). dct expression was significantly decreased in the ventral RPE of otx1a/otx2 morphants and to an even greater degree in otx1a/otx1b/otx2 morphants (E–F, white arrows). dct expression in neural crest melanocytes was unaffected (black arrows). Approximately 50 larvae were examined for each condition.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dct WT control Prim-5 neural crest melanocyte ISH
Prim-5 optic cup ventral-most region ISH
WT + MO1-otx1 standard conditions Prim-5 neural crest melanocyte ISH
Prim-5 optic cup ventral-most region ISH
WT + MO1-otx1 + MO1-otx2a + MO1-otx2b standard conditions Prim-5 neural crest melanocyte ISH
Prim-5 Not Detected presumptive retinal pigmented epithelium ventral-most region ISH
WT + MO1-otx2a standard conditions Prim-5 neural crest melanocyte ISH
Prim-5 optic cup ventral-most region ISH
WT + MO1-otx2a + MO1-otx2b standard conditions Prim-5 neural crest melanocyte ISH
Prim-5 Not Detected presumptive retinal pigmented epithelium ventral-most region ISH
WT + MO1-otx2b standard conditions Prim-5 neural crest melanocyte ISH
Prim-5 optic cup ventral-most region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One