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ZFIN ID: ZDB-FIG-121212-25
Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development. PLoS One   7(11):e49357 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 2 Otx morphant phenotypes.

(A–C) Eye phenotypes, assessed at 5 dpf, were scored as normal when the RPE displayed no visible defects (A), minor when coloboma was present but anterior and posterior ventral RPE were still contiguous (B), and major when the anterior and posterior ventral RPE were completely separated (C). (D) Histogram showing the eye defects induced by otx gene knockdown when injected at a morpholino concentration of 1.25 ng/embryo. Knockdown of individual otx genes caused only minor defects, while knockdown of otx1a and otx2 together, or all three otx genes together, produced a majority of embryos with major eye defects. otx1a, N = 108; otx1b, N = 230; otx2, N = 122; otx1a/otx1b, N = 222; otx1b/otx2, N = 256; otx1a/otx2, N = 214; otx1a/otx1b/otx2, N = 178.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-otx1 standard conditions Day 5 eye morphology, normal
WT + MO1-otx1 + MO1-otx2a standard conditions Day 5 eye perforate, abnormal
Day 5 optic fissure closure incomplete, abnormal
Day 5 retinal pigmented epithelium anterior side separated from retinal pigmented epithelium posterior side, abnormal
Day 5 retinal pigmented epithelium ventral region absent, abnormal
WT + MO1-otx1 + MO1-otx2a + MO1-otx2b standard conditions Day 5 retinal pigmented epithelium anterior side separated from retinal pigmented epithelium posterior side, abnormal
Day 5 retinal pigmented epithelium ventral region absent, abnormal
WT + MO1-otx1 + MO1-otx2b standard conditions Day 5 eye perforate, abnormal
Day 5 optic fissure closure incomplete, abnormal
WT + MO1-otx2a standard conditions Day 5 eye morphology, normal
WT + MO1-otx2a + MO1-otx2b standard conditions Day 5 eye perforate, abnormal
Day 5 optic fissure closure incomplete, abnormal
WT + MO1-otx2b standard conditions Day 5 eye morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One