ZFIN ID: ZDB-FIG-121212-28
Lane et al., 2012 - Otx but not mitf transcription factors are required for zebrafish retinal pigment epithelium development. PLoS One   7(11):e49357 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites

Fig. 5 mitfa and mitfb expression is decreased in otx morphants.

Expression of mitfa (A–D) and mitfb (E–H) was examined through in situ hybridization at the 21 somite stage in embryos injected with a control morpholino (A,E), or morpholinos against otx1a (B,F), otx1b (C,G), or otx2 (D,H). In situ hybridization analysis revealed a decrease in mitfa expression in the developing RPE cells (black arrows) but not in the neural crest cells (white arrows) of otx single morphants (D) and when compared to controls (A). mitfb expression was also reduced specifically in the RPE (black arrows) of otx morphants (F–H) compared to the controls, leaving epiphysis expression unaffected (white arrows). All morphants were processed simultaneously with approximately 50 larvae for each condition and the experiment was repeated twice.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mitfa WT control 20-25 somites neural crest cell ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx1 standard conditions 20-25 somites neural crest cell ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx2a standard conditions 20-25 somites neural crest cell ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx2b standard conditions 20-25 somites neural crest cell ISH
20-25 somites optic cup pigmented epithelial cell ISH
mitfb WT control 20-25 somites epiphysis ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx1 standard conditions 20-25 somites epiphysis ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx2a standard conditions 20-25 somites epiphysis ISH
20-25 somites optic cup pigmented epithelial cell ISH
WT + MO1-otx2b standard conditions 20-25 somites epiphysis ISH
20-25 somites optic cup pigmented epithelial cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One