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ZFIN ID: ZDB-FIG-120810-59
Lee et al., 2012 - Disruption of the folate pathway in zebrafish causes developmental defects. BMC Developmental Biology   12(1):12 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Shield to Prim-5

Fig. 5 Methotrexate–treated embryos maintain expression of appropriate developmental markers. Expression patterns were visualized via whole-mount in situ hybridization in embryos treated with 400 μM MTX and fixed at shield stage (A-H), tailbud (I-P), and 24 hpf (Q-W′). While MTX-treated embryos at 24hpf are clearly delayed with respect to normal development, they do express appropriate developmental markers: krt18 –enveloping layer, gsc – involuting cells and neural plate, ntl – blastomere margin, notochord and dorsal organizer, foxA2 – involuting cells and neural plate, crestin – neural crest, myoD – paraxial mesoderm, hgg- hatching gland (anterior most structure), ngn – CNS. (A-J) Animal view, dorsal to the right. (K-P) Lateral view with dorsal view inset. (Q, R, R′, T, V, W, X, X′) Dorsal view. (S, U, V′) Lateral view. (T′) ventral view.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crestin AB/TU standard conditions Prim-5 neural crest ISH
AB/TU chemical treatment: methotrexate Prim-5 neural crest ISH
ctslb AB/TU standard conditions Prim-5 hatching gland ISH
AB/TU chemical treatment: methotrexate Prim-5 hatching gland ISH
foxa2 AB/TU standard conditions Shield margin ISH
Bud neural plate ISH
AB/TU chemical treatment: methotrexate Shield margin ISH
Bud neural plate ISH
gsc AB/TU standard conditions Shield shield ISH
Bud neural plate ISH
AB/TU chemical treatment: methotrexate Shield shield ISH
Bud neural plate ISH
krt18a.1 AB/TU standard conditions Shield EVL ISH
Bud periderm ISH
AB/TU chemical treatment: methotrexate Shield EVL ISH
Bud periderm ISH
myod1 AB/TU standard conditions Prim-5 paraxial mesoderm ISH
AB/TU chemical treatment: methotrexate Prim-5 paraxial mesoderm ISH
neurog1 AB/TU standard conditions Prim-5 central nervous system ISH
AB/TU chemical treatment: methotrexate Prim-5 central nervous system ISH
tbxta AB/TU standard conditions Shield margin ISH
Shield shield ISH
Bud notochord ISH
Prim-5 notochord ISH
AB/TU chemical treatment: methotrexate Shield margin ISH
Shield shield ISH
Bud notochord ISH
Prim-5 notochord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.