ZFIN ID: ZDB-FIG-110407-6 |
Genes: | |
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Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage Range: | 1-4 somites to Prim-15 |
Fish: | |
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Knockdown Reagents: | |
Observed In: | |
Stage: | 1-4 somites |
Fig. 6 fgf24 is required for epibranchial development. (A, E) Expression of pax8 at 11 hpf in a control embryo (A) and fgf24 morphant (E). (B, F) Expression of sox3 at 14 hpf in a control embryo (B) and fgf24 morphant (F). White arrows indicate the lateral edges of the otic domain and black arrows indicate the lateral edges of the epibranchial domain. (C, G) Two color in situ hybridization showing pax2a (red) and foxi1 (blue) in a control embryo (C) and fgf24 morphant (G) at 14 hpf. Positions of otic placodes (op) are indicated. (D, H) Expression of phox2a at 30 hpf in a control embryo (D) and fgf24 morphant (H). Positions of the facial ganglion (f), glossopharyngeal ganglion (g) and vagal ganglia (v1 and v2) are indicated. Images show dorsolateral views with anterior to the left (A, D, E, H); dorsal views with anterior to the left and dorsal to the top (B, C, F, G). |
Gene | Antibody | Fish | Conditions | Stage | Qualifier | Anatomy | Assay |
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foxi1 | AB | control | 10-13 somites | epibranchial field | ISH | ||
AB + MO2-fgf24 + MO4-tp53 | standard conditions | 10-13 somites | epibranchial field | ISH | |||
pax2a | AB | control | 10-13 somites | otic placode | ISH | ||
AB + MO2-fgf24 + MO4-tp53 | standard conditions | 10-13 somites | otic placode | ISH | |||
pax8 | AB | control | 1-4 somites | otic placode | ISH | ||
AB + MO2-fgf24 + MO4-tp53 | standard conditions | 1-4 somites | otic placode | ISH | |||
phox2a | AB | control | Prim-15 | facial ganglion | ISH | ||
Prim-15 | glossopharyngeal ganglion | ISH | |||||
Prim-15 | vagal ganglion 1 | ISH | |||||
Prim-15 | vagal ganglion 2 | ISH | |||||
AB + MO2-fgf24 + MO4-tp53 | standard conditions | Prim-15 | facial ganglion | ISH | |||
Prim-15 | Not Detected | glossopharyngeal ganglion | ISH | ||||
Prim-15 | Not Detected | vagal ganglion 1 | ISH | ||||
Prim-15 | Not Detected | vagal ganglion 2 | ISH | ||||
sox3 | AB | control | 10-13 somites | epibranchial field | ISH | ||
AB + MO2-fgf24 + MO4-tp53 | standard conditions | 10-13 somites | otic placode | ISH |
Fish | Conditions | Stage | Phenotype |
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AB + MO2-fgf24 + MO4-tp53 | standard conditions | 1-4 somites | otic placode size, normal |
Acknowledgments: |
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol. |
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