ZFIN Figure: Padanad et al., 2011, Fig. 6

ZFIN ID: ZDB-FIG-110407-6

ZFIN ID: ZDB-FIG-110407-6

Padanad et al., 2011 - Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24. Developmental Biology 351(1):90-98 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	foxi1 pax2a pax8 phox2a sox3
Fish:	AB AB + MO2-fgf24 + MO4-tp53
Knockdown Reagents:	MO2-fgf24 MO4-tp53
Anatomical Terms:	epibranchial field facial ganglion glossopharyngeal ganglion otic placode vagal ganglion 1 vagal ganglion 2
Stage Range:	1-4 somites to Prim-15

PHENOTYPE:

Fish:	AB + MO2-fgf24 + MO4-tp53
Knockdown Reagents:	MO2-fgf24 MO4-tp53
Observed In:	otic placode
Stage:	1-4 somites

Fig. 6 fgf24 is required for epibranchial development. (A, E) Expression of pax8 at 11 hpf in a control embryo (A) and fgf24 morphant (E). (B, F) Expression of sox3 at 14 hpf in a control embryo (B) and fgf24 morphant (F). White arrows indicate the lateral edges of the otic domain and black arrows indicate the lateral edges of the epibranchial domain. (C, G) Two color in situ hybridization showing pax2a (red) and foxi1 (blue) in a control embryo (C) and fgf24 morphant (G) at 14 hpf. Positions of otic placodes (op) are indicated. (D, H) Expression of phox2a at 30 hpf in a control embryo (D) and fgf24 morphant (H). Positions of the facial ganglion (f), glossopharyngeal ganglion (g) and vagal ganglia (v₁ and v₂) are indicated. Images show dorsolateral views with anterior to the left (A, D, E, H); dorsal views with anterior to the left and dorsal to the top (B, C, F, G).

Gene Expression Details

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay
foxi1	AB	control	10-13 somites		epibranchial field	ISH
	AB + MO2-fgf24 + MO4-tp53	standard conditions	10-13 somites		epibranchial field	ISH
pax2a	AB	control	10-13 somites		otic placode	ISH
	AB + MO2-fgf24 + MO4-tp53	standard conditions	10-13 somites		otic placode	ISH
pax8	AB	control	1-4 somites		otic placode	ISH
	AB + MO2-fgf24 + MO4-tp53	standard conditions	1-4 somites		otic placode	ISH
phox2a	AB	control	Prim-15		facial ganglion	ISH
			Prim-15		glossopharyngeal ganglion	ISH
			Prim-15		vagal ganglion 1	ISH
			Prim-15		vagal ganglion 2	ISH
	AB + MO2-fgf24 + MO4-tp53	standard conditions	Prim-15		facial ganglion	ISH
			Prim-15	Not Detected	glossopharyngeal ganglion	ISH
			Prim-15	Not Detected	vagal ganglion 1	ISH
			Prim-15	Not Detected	vagal ganglion 2	ISH
sox3	AB	control	10-13 somites		epibranchial field	ISH
	AB + MO2-fgf24 + MO4-tp53	standard conditions	10-13 somites		otic placode	ISH

Antibody Labeling Details No data available

Phenotype Details

Fish	Conditions	Stage	Phenotype
AB + MO2-fgf24 + MO4-tp53	standard conditions	1-4 somites	otic placode size, normal

Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.