ZFIN ID: ZDB-FIG-110407-6
Padanad et al., 2011 - Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24. Developmental Biology   351(1):90-98 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 1-4 somites

Fig. 6 fgf24 is required for epibranchial development. (A, E) Expression of pax8 at 11 hpf in a control embryo (A) and fgf24 morphant (E). (B, F) Expression of sox3 at 14 hpf in a control embryo (B) and fgf24 morphant (F). White arrows indicate the lateral edges of the otic domain and black arrows indicate the lateral edges of the epibranchial domain. (C, G) Two color in situ hybridization showing pax2a (red) and foxi1 (blue) in a control embryo (C) and fgf24 morphant (G) at 14 hpf. Positions of otic placodes (op) are indicated. (D, H) Expression of phox2a at 30 hpf in a control embryo (D) and fgf24 morphant (H). Positions of the facial ganglion (f), glossopharyngeal ganglion (g) and vagal ganglia (v1 and v2) are indicated. Images show dorsolateral views with anterior to the left (A, D, E, H); dorsal views with anterior to the left and dorsal to the top (B, C, F, G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxi1 AB control 10-13 somites epibranchial field ISH
AB + MO2-fgf24 + MO4-tp53 standard conditions 10-13 somites epibranchial field ISH
pax2a AB control 10-13 somites otic placode ISH
AB + MO2-fgf24 + MO4-tp53 standard conditions 10-13 somites otic placode ISH
pax8 AB control 1-4 somites otic placode ISH
AB + MO2-fgf24 + MO4-tp53 standard conditions 1-4 somites otic placode ISH
phox2a AB control Prim-15 facial ganglion ISH
Prim-15 glossopharyngeal ganglion ISH
Prim-15 vagal ganglion 1 ISH
Prim-15 vagal ganglion 2 ISH
AB + MO2-fgf24 + MO4-tp53 standard conditions Prim-15 facial ganglion ISH
Prim-15 Not Detected glossopharyngeal ganglion ISH
Prim-15 Not Detected vagal ganglion 1 ISH
Prim-15 Not Detected vagal ganglion 2 ISH
sox3 AB control 10-13 somites epibranchial field ISH
AB + MO2-fgf24 + MO4-tp53 standard conditions 10-13 somites otic placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-fgf24 + MO4-tp53 standard conditions 1-4 somites otic placode size, normal
Acknowledgments:
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Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.