ZFIN ID: ZDB-FIG-110407-3
Padanad et al., 2011 - Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24. Developmental Biology   351(1):90-98 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 3 Requirement for pax2/8 in otic and epibranchial development. (A, E) Expression of sox3 at 13 hpf in a control embryo (A) and pax8 morphant (E). White arrows indicate the lateral edges of the otic domain and black arrows indicate the edges of the epibranchial domain. (B, C, F, G) Two color in situ hybridization of embryos at 13 hpf (B, F) and 24 hpf (C, G) showing expression of pax2a (red) and foxi1 (blue). Outlines indicate the otic vesicle (C) or vestigial otic region (G). Expression patterns are shown in control embryos (B, C) and pax2a/pax2b/pax8-deficient embryos (F, G). (D, H) Expression of phox2a at 30 hpf in a control embryo (D) and pax2a/2b/8-deficient embryo (H). Images show dorsal views with anterior to the top (A–C, E–G); dorsolateral views with anterior to the left and dorsal to the top (D, H). Positions of the facial ganglion (f), glossopharyngeal ganglion (g) and vagal ganglia (v1 and v2) are indicated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
foxi1 AB control 5-9 somites epibranchial field ISH
Prim-5 epibranchial placode ISH
pax2atu29a/tu29a + MO1-pax2b + MO6-pax8 + MO7-pax8 standard conditions 5-9 somites epibranchial field ISH
5-9 somites otic placode ISH
Prim-5 epibranchial placode ISH
Prim-5 otic vesicle ISH
pax2a AB control 5-9 somites otic placode ISH
Prim-5 otic vesicle ISH
pax2atu29a/tu29a + MO1-pax2b + MO6-pax8 + MO7-pax8 standard conditions 5-9 somites otic placode ISH
Prim-5 otic vesicle ISH
phox2a AB control Prim-15 facial ganglion ISH
Prim-15 glossopharyngeal ganglion ISH
Prim-15 vagal ganglion 1 ISH
Prim-15 vagal ganglion 2 ISH
pax2atu29a/tu29a + MO1-pax2b + MO6-pax8 + MO7-pax8 standard conditions Prim-15 facial ganglion ISH
Prim-15 glossopharyngeal ganglion ISH
Prim-15 Not Detected vagal ganglion 1 ISH
Prim-15 Not Detected vagal ganglion 2 ISH
sox3 AB control 5-9 somites epibranchial field ISH
AB + MO6-pax8 + MO7-pax8 standard conditions 5-9 somites otic placode ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
pax2atu29a/tu29a + MO1-pax2b + MO6-pax8 + MO7-pax8 standard conditions Prim-15 epibranchial ganglion decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.