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ZFIN ID: ZDB-FIG-110128-47
Ríos et al., 2011 - Zebrafish usp39 Mutation Leads to rb1 mRNA Splicing Defect and Pituitary Lineage Expansion. PLoS Genetics   7(1):e1001271 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 3 usp39 mutation lead to expansion of all pituitary cell lineages at 48 hpf as indicated by pituitary hormone markers.

A–L: Whole-mount double in situ hybridization with probes indicated on the side. usp39 mutant embryos exhibit higher expression of all pituitary hormone markers compared to wild-type (wt) embryos. Spatial distribution of prl, tsh, pomc and cga are normal in the usp39 mutant. (A, B, E, F, I, J, M, and N) ventral view and (C, D, G, H, K, L, O, and P) lateral view, with anterior to the left. Columns 1 (A, E, I, and M) and 3 (C, G, K and O) show wt siblings; columns 2 (B, F, J, and N) and 4 (D, H, L, and P) show usp39 mutant embryos. A–D: gh (purple) and prl (red) transcripts. (C) The spatial distribution of gh is normally found in the proximal pars distalis (white arrow) and prl is found in the rostral pars distalis (black arrow). (D) Note the spatial distribution of gh in the usp39 mutant; gh is abnormally expressed in the rostral pars distalis (black arrow). E–H: tsh (purple) and pomc (red) transcripts. I–L: prl (purple) and pomc (red) transcripts. M–P: Whole-mount in situ hybridization with cga transcript.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cga WT standard conditions Long-pec pars anterior ISH
usp39zf232/zf232 standard conditions Long-pec pars anterior ISH
gh1 WT standard conditions Long-pec proximal pars anterior ISH
usp39zf232/zf232 standard conditions Long-pec rostral pars anterior ISH
prl WT standard conditions Long-pec rostral pars anterior ISH
usp39zf232/zf232 standard conditions Long-pec rostral pars anterior ISH
tshba WT standard conditions Long-pec pars anterior ISH
usp39zf232/zf232 standard conditions Long-pec pars anterior ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
usp39zf232/zf232 standard conditions Long-pec proximal pars anterior morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.