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ZIRC
ZFIN ID: ZDB-FIG-101101-4
Ablooglu et al., 2010 - Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry. Development (Cambridge, England)   137(20):3449-3458 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 75%-epiboly to Prim-15

Fig. 4 Integrin β1b morphants phenocopy the organ asymmetry defects of αV morphants. (A,B) Dorsal views of wild-type embryos at 80% E, hybridized with a probe to β1b and β5. Putative DFCs are positive for β1b transcripts (black arrow; A), and β5 transcripts are present in the marginal cells where there is a gap in this expression field (black arrow, B). (C,D) Double WISH samples showing simultaneous expression of cas (red orange) and β1b (dark purple; C); or cas (red orange) and β5 (dark purple; D). (E-H) Dorsal views of embryos, slightly tilted to visualize cas (E) and ntl (G) expression in DFCs. Delivery of β1b MOs to 1- to 4-cell-stage embryos caused improper formation of forerunner cell cluster, assessed by cas (E) and ntl (G) expression in DFCs at 80% E. DFCs in β1b morphants had occasional gaps and were separated from the axis (black arrow in G). (F,H) Bar graphs summarize DFC phenotypes using cas or ntl as markers. Phenotypic classification of DFCs and data analyses were similar to those in Fig. 3. (I,J) β1b morphants developed undulated tail and abnormal head phenotype, which were evident at 32 hpf. (K) spaw expression in β1b morphants in lateral plate mesoderm (LPM) in 15-17 somite stage (SS) embryos. Dorsal view of a representative β1bEI10 morphant (5 ng) showing right-sided spaw in LPM (arrowhead). (L) Bar graph showing the effects of β1b integrin loss-of-function on localization of spaw expression. (M) When β1bEI10 was injected into yolk at mid-blastula stage (512-1000 cells), animals had wild-type phenotype. (N) A representative fluorescence image corresponding to M that shows MO presence only in the yolk cell. (O) Bar graph showing effects of β1b integrin loss selectively in DFCs on heart tube location. Data expressed are similar to those in Fig. 1E. Scale bars: 50 μm in A-D; 20 μm in E; 100 μm in G,K; 500 μm in I,J,M,N.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
itgb1b WT standard conditions 75%-epiboly axis ISH
75%-epiboly forerunner cell group ISH
itgb5 WT standard conditions 75%-epiboly Not Detected forerunner cell group ISH
75%-epiboly margin ISH
sox32 WT standard conditions 75%-epiboly forerunner cell group ISH
WT + MO2-itgb1b standard conditions 75%-epiboly forerunner cell group ISH
WT + MO3-itgb1b standard conditions 75%-epiboly forerunner cell group ISH
spaw WT + MO2-itgb1b standard conditions 14-19 somites lateral plate mesoderm right side ISH
WT + MO3-itgb1b standard conditions 14-19 somites lateral plate mesoderm right side ISH
tbxta WT + MO2-itgb1b standard conditions 75%-epiboly axis ISH
75%-epiboly forerunner cell group ISH
WT + MO3-itgb1b standard conditions 75%-epiboly axis ISH
75%-epiboly forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-itgb1b standard conditions 75%-epiboly forerunner cell group spatial pattern, abnormal
14-19 somites determination of left/right asymmetry in lateral mesoderm disrupted, abnormal
Prim-15 head morphology, abnormal
Prim-15 pericardium edematous, abnormal
Prim-15 post-vent region undulate, abnormal
Prim-15 somite U-shaped, abnormal
Prim-15 ventricular system increased accumulation cerebral spinal fluid, abnormal
WT + MO3-itgb1b standard conditions 75%-epiboly forerunner cell group spatial pattern, abnormal
14-19 somites determination of left/right asymmetry in lateral mesoderm disrupted, abnormal
Prim-15 head morphology, abnormal
Prim-15 pericardium edematous, abnormal
Prim-15 post-vent region undulate, abnormal
Prim-15 somite U-shaped, abnormal
Prim-15 ventricular system increased accumulation cerebral spinal fluid, abnormal
WT + MO3-itgb1b standard conditions Prim-5 determination of heart left/right asymmetry disrupted, abnormal
Prim-5 heart tube positional polarity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development