PUBLICATION
Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry
- Authors
- Ablooglu, A.J., Tkachenko, E., Kang, J., and Shattil, S.J.
- ID
- ZDB-PUB-100929-2
- Date
- 2010
- Source
- Development (Cambridge, England) 137(20): 3449-3458 (Journal)
- Registered Authors
- Ablooglu, Ararat
- Keywords
- Alpha V, Beta 1b, Dorsal forerunner cells, Gastrulation, Integrin, Zebrafish
- MeSH Terms
-
- Animals
- Blastoderm/cytology*
- Blastoderm/physiology
- Blotting, Western
- Body Patterning/physiology*
- Cloning, Molecular
- DNA Primers/genetics
- Gastrulation/physiology*
- Gene Knockdown Techniques
- Immunohistochemistry
- Integrin alphaV/genetics
- Integrin alphaV/metabolism*
- Integrin beta1/metabolism
- Zebrafish/embryology*
- PubMed
- 20843856 Full text @ Development
Citation
Ablooglu, A.J., Tkachenko, E., Kang, J., and Shattil, S.J. (2010) Integrin alphaV is necessary for gastrulation movements that regulate vertebrate body asymmetry. Development (Cambridge, England). 137(20):3449-3458.
Abstract
Integrin αV can form heterodimers with several β subunits to mediate cell-cell and cell-extracellular matrix interactions. During zebrafish gastrulation, αV is expressed maternally and zygotically. Here, we used a morpholino-mediated αV knockdown strategy to study αV function. Although αV morphants displayed vascular defects, they also exhibited left-right body asymmetry defects affecting multiple visceral organs. This was preceded by mislocalization of dorsal forerunner cells (DFCs) and malformation of the Kupffer's vesicle (KV) laterality organ. These defects were rescued with morpholino-resistant αV mRNA. Like αV, integrin β1b was expressed in DFCs, and β1b knockdown largely recapitulated the laterality phenotype of αV morphants. When tracked in real-time, individual DFCs of both morphants showed defects in DFC migration, preventing them from organizing into a KV of normal shape and size. Thus, we propose that αVβ1b mediates cellular interactions that are necessary for DFC clustering and movements necessary for Kupffer's vesicle formation, uncovering an early contribution of integrins to the regulation of vertebrate laterality.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping