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ZFIN ID: ZDB-FIG-100603-4
Zuniga et al., 2010 - Jagged-Notch signaling ensures dorsal skeletal identity in the vertebrate face. Development (Cambridge, England)   137(11):1843-1852 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 4 Expression of jag1b, notch2 and hey1 is regulated by Jagged-Notch and Edn1 signaling. (A-N) Confocal sections of double-fluorescence in situ hybridizations showing mandibular (m) and hyoid (h) arch expression of jag1b (green) and notch2 (red) (A-G) and of dlx2a (green) and hey1 (red) (H-N). In wild types, jag1b and hey1 CNCC expression expands ventrally from 28 hpf (A,H) to 32 hpf (B,I) and 36 hpf (C,J). In jag1bb1105 (D,K) and notch2-MO (E,L) zebrafish embryos, jag1b and hey1 expression is reduced and strong ventral notch2 expression (brackets) is expanded at 36 hpf. Arrowheads in J,K indicate ventral mesoderm expression of hey1, which is unaffected in jag1bb1105 mutants. In 20-28 hpf heat shock-treated hsp70I:Gal4; UAS:JAG1 (F,M) and edn1-/- (G,N) embryos, jag1b and hey1 expression is expanded into ventral CNCCs (arrows) and notch2 expression is reduced at 36 hpf. Endodermal pouches are outlined in A-G.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch 2 skeleton ISH
Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
edn1tf216b/tf216b standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
hey1 WT standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch 2 skeleton ISH
Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 pharyngeal arch 2 skeleton ISH
edn1tf216b/tf216b standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1b WT standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch 2 skeleton ISH
Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 Not Detected mandibular arch skeleton ISH
Prim-25 Not Detected pharyngeal arch 2 skeleton ISH
edn1tf216b/tf216b standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
el108Tg; kca4Tg/+ control Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 Not Detected mandibular arch skeleton ISH
Prim-25 Not Detected pharyngeal arch 2 skeleton ISH
notch2 WT standard conditions Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch 2 skeleton ISH
Prim-15 mandibular arch skeleton ISH
Prim-15 pharyngeal arch 2 skeleton ISH
Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
WT + MO1-notch2 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
edn1tf216b/tf216b standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
el108Tg; kca4Tg/+ heat shock Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
jag1bb1105/b1105 standard conditions Prim-25 mandibular arch skeleton ISH
Prim-25 pharyngeal arch 2 skeleton ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development