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ZIRC
ZFIN ID: ZDB-FIG-100603-11
Zuniga et al., 2010 - Jagged-Notch signaling ensures dorsal skeletal identity in the vertebrate face. Development (Cambridge, England)   137(11):1843-1852 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 6 Reduction of Jag1b-Notch2 signaling rescues the ventral defects of edn1 mutants. (A-F) Ventral views of dissected zebrafish facial skeletons at 5 dpf, with elements labeled in wild type (A). jag1bb1105 mutants (B) have Pq reductions (arrowhead) and variable transformations of Hm (arrow) and Op (asterisk). In edn1-/- mutants (C), M (red arrow) and Ch (red arrowhead) are nearly absent. In edn1-/-; jag1bb1105 larvae (D), development of ventral M and Ch is variably restored yet Pq and Hm defects are still evident. M and Ch development is also partially restored in some edn1-/-; jag1bb1105/+ (E) and edn1-/-; notch2-MO (F) larvae. (G) Quantification of skeletal rescue, showing wild-type (yellow), weakly defective (red), severely defective (blue), and expanded (green) cartilage and bone. (H) In situ hybridizations showing dlx3b, dlx5a and dlx6a expression in arch CNCCs at 36 hpf. Compared with edn1-/- embryos, edn1-/-; jag1bb1105 embryos show partial rescue of expression. Endodermal pouches are outlined. See Fig. 1 for abbreviations.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
dlx5a WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
dlx6a WT standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Day 5 branchiostegal ray decreased size, abnormal
Day 5 ceratohyal cartilage absent, abnormal
Day 5 ceratohyal cartilage decreased size, abnormal
Day 5 Meckel's cartilage absent, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 opercle increased variability of size, abnormal
Day 5 symplectic absent, abnormal
Day 5 symplectic decreased size, abnormal
edn1tf216b/tf216b + MO1-notch2 standard conditions Day 5 ceratohyal cartilage decreased size, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 Meckel's cartilage morphology, abnormal
Day 5 symplectic decreased size, abnormal
edn1tf216b/tf216b; jag1bb1105/+ standard conditions Day 5 ceratohyal cartilage decreased size, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 Meckel's cartilage morphology, abnormal
Day 5 symplectic decreased size, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 ceratohyal cartilage decreased size, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 Meckel's cartilage decreased size, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 Meckel's cartilage morphology, abnormal
edn1tf216b/tf216b; jag1bb1105/b1105 standard conditions Day 5 symplectic decreased size, abnormal
jag1bb1105/b1105 standard conditions Day 5 hyosymplectic cartilage deformed, abnormal
Day 5 mandibular arch skeleton morphology, abnormal
Day 5 opercle decreased width, abnormal
Day 5 palatoquadrate cartilage truncated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development