ZFIN ID: ZDB-FIG-100429-8
Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling. Developmental Biology   340(2):293-305 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 2 The neural tube is ventralized in Rest knockdown embryos. Transverse sections of 29hpf (A, B) or 26hpf (C–H) wild-type embryos processed for RNA in situ hybridization and sectioned at the level of the hindbrain at anterior rhombomere 4. Control (A, C, E, G) and rest MO injected (B, D, F, H) embryos stained with antisense probes for Hh response genes pax3a, nkx2.2a, foxA2 and ptc1. pax3a expression (A, B) is reduced in rest morphants, while expression of nkx2.2a (C, D), foxA2 (E, F) and ptc1 (G, H) is expanded compared to stage matched control embryos. This suggests that Rest represses Hh signaling. Arrows (E, F) mark pharyngeal endoderm. (I) qPCR analysis on 29hpf control (red bars) and stage matched rest morphant (blue bars) cDNA for markers shown in A–H, plus class I gene dbx1a. Overall levels of class II Hh target genes nkx2.2a, foxA2, and ptc1 are increased while class I genes pax3a and dbx1a levels are reduced.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a WT control Prim-5 whole organism RTPCR
WT + MO1-rest standard conditions Prim-5 whole organism RTPCR
foxa2 WT control Prim-5 hindbrain neural tube ventral region ISH
Prim-5 pharyngeal endoderm ISH
Prim-5 whole organism RTPCR
WT + MO1-rest standard conditions Prim-5 hindbrain neural tube ventral region ISH
Prim-5 pharyngeal endoderm ISH
Prim-5 whole organism RTPCR
nkx2.2a WT control Prim-5 hindbrain neural tube ventral region ISH
Prim-5 whole organism RTPCR
WT + MO1-rest standard conditions Prim-5 hindbrain neural tube ventral region ISH
Prim-5 whole organism RTPCR
pax3a WT control Prim-5 hindbrain neural tube dorsal region ISH
Prim-5 whole organism RTPCR
WT + MO1-rest standard conditions Prim-5 hindbrain neural tube dorsal region ISH
Prim-5 whole organism RTPCR
ptch2 WT control Prim-5 neural tube ISH
Prim-5 whole organism RTPCR
WT + MO1-rest standard conditions Prim-5 neural tube ISH
Prim-5 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-rest standard conditions Prim-5 dorsal/ventral neural tube patterning disrupted, abnormal
Prim-5 neural tube wholly ventralized, abnormal
Prim-5 regulation of smoothened signaling pathway disrupted, abnormal
Prim-5 smoothened signaling pathway involved in dorsal/ventral neural tube patterning disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.