ZFIN ID: ZDB-FIG-100429-11
Gates et al., 2010 - The transcriptional repressor REST/NRSF modulates hedgehog signaling. Developmental Biology   340(2):293-305 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-5

Fig. 4 CyA mediated attenuation of Hh signaling is enhanced by Rest knockdown. Lateral views of 26 hpf control (A, C, E, G) and rest morphants (B, D, F, H) embryos stained for nkx2.2a (A–D) or pax3a (E–H). Embryos were incubated in control media (A, B, E, F) or 1.5 μM cyclopamine (CyA) media from 6 hpf on (C, D, G, H). rest morphants incubated in control media show a modest increase in nkx2.2a expression (B, compare to control, A) and a modest decrease in pax3a (F, compare to control, E) grown under the same conditions. 1.5 μM CyA decreases nkx2.2a expression (C) and modestly expands pax3a (G) in control embryos. Rest knockdown produces a greater reduction in nkx2.2a expression, and a greater expansion of pax3a, then CyA treatment alone.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.2a WT control Prim-5 hindbrain neural tube ventral region ISH
Prim-5 spinal cord neural tube ventral region ISH
WT chemical treatment: Cyclopamine Prim-5 hindbrain neural tube ventral region ISH
Prim-5 spinal cord neural tube ventral region ISH
WT + MO1-rest chemical treatment: Cyclopamine Prim-5 Not Detected hindbrain neural tube ventral region ISH
Prim-5 Not Detected neural tube ventral region ISH
WT + MO1-rest standard conditions Prim-5 hindbrain neural tube ventral region ISH
Prim-5 spinal cord neural tube ventral region ISH
pax3a WT control Prim-5 hindbrain neural tube dorsal region ISH
WT chemical treatment: Cyclopamine Prim-5 hindbrain neural tube dorsal region ISH
WT + MO1-rest chemical treatment: Cyclopamine Prim-5 hindbrain neural tube dorsal region ISH
WT + MO1-rest standard conditions Prim-5 hindbrain neural tube dorsal region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 340(2), Gates, K.P., Mentzer, L., Karlstrom, R.O., and Sirotkin, H.I., The transcriptional repressor REST/NRSF modulates hedgehog signaling, 293-305, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.