ZFIN ID: ZDB-FIG-090814-23
Viktorin et al., 2009 - Emx3 is required for the differentiation of dorsal telencephalic neurons. Developmental dynamics : an official publication of the American Association of Anatomists   238(8):1984-1998 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: emx3, foxg1a
Antibody: Ab1-tuba
Fish: WT + MO2-emx3 + MO3-emx3 + MO4-tp53, WT + MO4-tp53
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Anatomical Terms: telencephalon, telencephalon axon
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish: WT + MO2-emx3 + MO3-emx3 + MO4-tp53
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Observed In: anterior commissure, axon extension, axonal fasciculation, cell proliferation in forebrain, cranial nerve I (all 9) expand
Stage Range: Prim-5 to Long-pec

Fig. 5 Embryos injected with emx3 morpholino antisense oligonucleotides (MO) form abnormal axon tracts, lack olfactory glomeruli, and have normal cell division patterns. A-D: Acetylated tubulin labeling of axons; 35 hr; emx3 knockdown embryos lack anterior commissure (AC in B,D). The supraoptic tract (SOT) is present but not fasciculated (arrows in A,B). Similarly, the olfactory nerve (on) that extends from the developing olfactory organ (o) to the dorsal telencephalon (t) is defasciculated in emx3 knockdown embryos (arrows in C,D). E,F: emx3MO injected embryos have fewer presumptive olfactory glomeruli (yellow asterisks), visualized by Bodipy-ceramide staining. As in (G,H), the characteristic morphology of the ventricular zone appears broader in emx3MO embryos. G,H: Five minutes of 5-bromo-2-deoxyuridine (BrdU, white) incubation labels cells in S-phase. In control- and emx3MO-injected embryos at 24 hr, BrdU labeled cells are located in a 3-4 cell-wide region close to the ventricular zone. Mitotic cells, labeled by anti-phosphorylated histone H3 (pH3, green), are located on the ventricular side of the telencephalon in both tp53MO and emx3MO injected embryos. I-K: The number (K) and dorsoventral distribution (I-K) of pH3 positive cells in the medial telencephalic ventricular zone is the same in control (I,K) and emx3MO injected embryos (J,K). I,J: Projections of the side view confocal stack used for cell counts in K; the dotted line indicates the border between dorsal and ventral telencephalon, error bars denote SEM. Anterior is to the left, dorsal to the top. Confocal projections of oblique (A,B) straight (C-H) frontal views, dorsal to the top. os, optic stalk; POC, postoptic commissure. Scale bars = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
emx3 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 telencephalon ISH
foxg1a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 telencephalon ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-15 telencephalon axon
IHC WT + MO4-tp53 standard conditions Prim-15 telencephalon axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 cell proliferation in forebrain occurrence, normal
Prim-5 telencephalon ventricular zone increased width, abnormal
Prim-15 anterior commissure aplastic, abnormal
Prim-15 axon extension disrupted, abnormal
Prim-15 axonal fasciculation disrupted, abnormal
Prim-15 cranial nerve I defasciculated, abnormal
Prim-15 supraoptic tract decreased thickness, abnormal
Prim-15 supraoptic tract fasciculation, abnormal
Long-pec glomerular layer decreased size, abnormal
Long-pec olfactory bulb development disrupted, abnormal
Long-pec telencephalon ventricular zone increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.