ZFIN ID: ZDB-FIG-090814-22
Viktorin et al., 2009 - Emx3 is required for the differentiation of dorsal telencephalic neurons. Developmental dynamics : an official publication of the American Association of Anatomists   238(8):1984-1998 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: dlx2a, elavl3, elavl4, emx1, eomesa (all 19) expand
Fish: WT + MO2-emx3 + MO3-emx3 + MO4-tp53, WT + MO4-tp53
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Anatomical Terms: diencephalon, dorsal telencephalon, telencephalon, telencephalon diencephalon boundary, ventral telencephalon
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish: WT + MO2-emx3 + MO3-emx3 + MO4-tp53
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Observed In: dorsal telencephalon, forebrain neuron differentiation
Stage: Prim-5

Fig. 4 A-R: emx3 morpholino injection specifically reduces expression of dorsal telencephalic marker genes. A-D′,G-N′: Expression of tcf4l (A-A′) in the dorsal telencephalon is lost, and expression domains of atoh2b (B-B′), neurod (C-C′), elavl4 (D-D′), slc17a6 (G-G′) slc17a6l (H-H′), dlx2a (I-I′, only the domain between asterisks), lhx5 (J-J′), pax6a (K-K′), emx1 (L-L′), are reduced in emx3 morpholino antisense oligonucleotides (MO) injected embryos compared with control embryos. More broadly expressed neuronal differentiation markers elavl3 (E-E′) and reln (F-F′) show little or no apparent difference between controls and emx3MO injected embryos. fezf2 (M-M′) expression is reduced only in the more intense expression domain adjacent to the olfactory placodes (dotted lines), but unchanged in the ventricular zone. Ventral telencephalic expression domains (dlx2a, I-I′, fzd8a, N-N′; fgf8a, Q-Q′; foxg1, R-R′) are unaffected by emx3MO injections, as are diencephalic expression domains (C-K′, M-Q′). Shape and size of the telencephalon appears unaltered in emx3MO embryos compared with control embryos, as indicated for example by elavl3 (E-E′; t, telencephalon, outlined by white dotted lines; e, epiphysis; d, diencephalon), and foxg1 (R-R′) expression. Commonly used previous gene names of recently renamed genes are given in parentheses. A-B′, D-D′, G-H′, M-M′: Frontal views, dorsal to the top. C-C′, E-F′, I-L′, N-R′: Side views, dorsal to the top, rostral to the left; eyes removed except in Q-Q′. A-P′, R-R′: At 24 hr, (Q-Q′) 10-somite stage (14 hr). Scale bar = 100 ′m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
Prim-5 ventral telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
Prim-5 ventral telencephalon ISH
elavl3 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon ISH
elavl4 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
emx1 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
eomesa WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
fezf2 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
fgf8a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions 10-13 somites diencephalon ISH
10-13 somites telencephalon ISH
10-13 somites ventral telencephalon ISH
WT + MO4-tp53 standard conditions 10-13 somites diencephalon ISH
10-13 somites telencephalon ISH
10-13 somites ventral telencephalon ISH
foxg1a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 telencephalon ISH
Prim-5 ventral telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 telencephalon ISH
Prim-5 ventral telencephalon ISH
fzd8a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 ventral telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 ventral telencephalon ISH
lhx5 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
neurod1 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
neurod6b WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
pax6a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
reln WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
slc17a6a WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
slc17a6b WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 dorsal telencephalon ISH
tcf4 WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 Not Detected dorsal telencephalon ISH
WT + MO4-tp53 standard conditions Prim-5 dorsal telencephalon ISH
wnt7ba WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon diencephalon boundary ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon diencephalon boundary ISH
wnt7bb WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon diencephalon boundary ISH
WT + MO4-tp53 standard conditions Prim-5 diencephalon ISH
Prim-5 telencephalon diencephalon boundary ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-emx3 + MO3-emx3 + MO4-tp53 standard conditions Prim-5 dorsal telencephalon morphology, abnormal
Prim-5 forebrain neuron differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.