ZFIN ID: ZDB-FIG-090814-21
Viktorin et al., 2009 - Emx3 is required for the differentiation of dorsal telencephalic neurons. Developmental dynamics : an official publication of the American Association of Anatomists   238(8):1984-1998 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: eomesa, tcf4
Fish: AB + MO2-emx3 + MO3-emx3 + MO4-tp53, b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB), b1202Tg/+ + MO4-tp53(AB)
Condition: heat shock
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Anatomical Term: dorsal telencephalon
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish: AB + MO2-emx3 + MO3-emx3 + MO4-tp53, b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB), b1202Tg/+ + MO4-tp53(AB)
Condition: heat shock
Knockdown Reagents: MO2-emx3, MO3-emx3, MO4-tp53
Observed In: dorsal telencephalon, telencephalon
Stage Range: 10-13 somites to Prim-5

Fig. 6 A-H: Heat shock-induced Emx3 protein partially rescues emx3 morpholino antisense oligonucleotides (MO) -induced knockdown, whereas excessive Emx3 dorsalizes the telencephalon. A-C,E-G: Low levels of Emx3-myc-GFP expression (e3-GFP+), induced by heat shock at the 1- or 2-somite stages (10.5 hr, hs2s), 20-30 min after the onset of endogenous emx3 mRNA expression at bud stage, rescues the emx3MO-induced down regulation of tcf4l (A-C) and eomesa (eom., E-G). Later heat shock, between the 5- and 7-somite stages (11.5 hr, hs5s), does not rescue the phenotype (B,D). Higher levels of GFP expression after heat shock (e3-GFP++), indicating higher levels of Emx3 activity, lead to dorsalized telencephalon as assessed by ventrally expanded expression of tcf4l (A,H). A-D,H: Frontal views, dorsal to the top, 25 hr; (E-G) Dorsal view, rostral to the left, 12-somite stage (15 hr). Scale bar = 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
eomesa AB + MO2-emx3 + MO3-emx3 + MO4-tp53 heat shock 10-13 somites dorsal telencephalon ISH
b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB) heat shock 10-13 somites dorsal telencephalon ISH
b1202Tg/+ + MO4-tp53(AB) heat shock 10-13 somites dorsal telencephalon ISH
tcf4 AB + MO2-emx3 + MO3-emx3 + MO4-tp53 heat shock Prim-5 Not Detected dorsal telencephalon ISH
b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB) heat shock Prim-5 dorsal telencephalon ISH
b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB) heat shock Prim-5 Not Detected dorsal telencephalon ISH
b1202Tg/+ + MO4-tp53(AB) heat shock Prim-5 dorsal telencephalon ISH
b1202Tg/+ + MO4-tp53(AB) heat shock Prim-5 dorsal telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-emx3 + MO3-emx3 + MO4-tp53 heat shock 10-13 somites dorsal telencephalon morphology, abnormal
Prim-5 dorsal telencephalon morphology, abnormal
b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB) heat shock 10-13 somites dorsal telencephalon morphology, normal
Prim-5 dorsal telencephalon morphology, normal
b1202Tg/+ + MO2-emx3 + MO3-emx3 + MO4-tp53(AB) heat shock Prim-5 dorsal telencephalon morphology, abnormal
b1202Tg/+ + MO4-tp53(AB) heat shock Prim-5 telencephalon wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.