ZFIN ID: ZDB-FIG-090501-18
Esaki et al., 2009 - Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae. Developmental Biology   329(1):116-129 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 75%-epiboly to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to Prim-5

Fig. 3 Broad expression of foxi1 in the presumptive ectoderm from 8 hpf and its regulatory role upstream of deltaC and foxi3a. (A–C) Whole mount in situ hybridization with foxi1 probe at 8 hpf (A), 9 hpf (B), and 12 hpf (C). (D–F) Whole mount in situ hybridization with deltaC probe at 12 hpf of uninjected wild-type control (D), foxi1 morphant (E), and foxi1 mRNA-injected embryos (F). (G, H) Whole mount in situ hybridization with notch1a probe at 12 hpf of uninjected (G) and foxi1 MO-injected (H) embryos. (I–U) Whole mount in situ hybridization with (i) foxi3a probe at 12 hpf (I, M, R) and 24 hpf (J, N, S), (ii) atp6v1a probe at 24 hpf (K, O, Q, T), and (iii) atp1a1a.2 probe at 24 hpf (L, P, U) of uninjected wild-type controls (I–L), foxi1 morphants (M–P), foxi1 morphant injected with foxi3a mRNA (Q), and foxi1 mRNA-injected embryos (R–U); for the controls of R–U, also see Figs. 2C, G, H. atp1a1a.2 and atp6v1a are marker genes for NaK-MRC and vH-MRC, respectively. Red arrow in panel U indicates foxi1-induced ectopic generation of NaK-MRC in the head region. Embryos are shown with their ventral side down and animal pole to the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atp1a1a.2 WT standard conditions Prim-5 extension integument ISH
Prim-5 trunk integument ISH
WT + MO3-foxi1 standard conditions Prim-5 extension integument ISH
Prim-5 trunk integument ISH
atp6v1ab WT standard conditions Prim-5 extension integument ISH
WT + MO3-foxi1 standard conditions Prim-5 Not Detected integument ISH
dlc WT standard conditions 5-9 somites integument ISH
5-9 somites somite ISH
WT + MO3-foxi1 standard conditions 5-9 somites Not Detected integument ISH
5-9 somites somite ISH
foxi1 WT standard conditions 75%-epiboly to 5-9 somites ectoderm ISH
foxi3a WT standard conditions 5-9 somites yolk integument ISH
Prim-5 extension integument ISH
WT + MO3-foxi1 standard conditions 5-9 somites Not Detected integument ISH
Prim-5 Not Detected integument ISH
notch1a WT standard conditions 5-9 somites whole organism ISH
WT + MO3-foxi1 standard conditions 5-9 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-foxi1 standard conditions 5-9 somites epithelial cell differentiation process quality, abnormal
WT + MO3-foxi1 standard conditions 5-9 somites integument physical object quality, abnormal
WT + MO3-foxi1 standard conditions Prim-5 epithelial cell differentiation process quality, abnormal
WT + MO3-foxi1 standard conditions Prim-5 integument physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 329(1), Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S., Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae, 116-129, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.