FIGURE

Fig. 3

ID

ZDB-FIG-080502-8

Publication

Sanek et al., 2008 - A novel role for zebrafish zic2a during forebrain development

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Fig. 3

Zic2a is required for diencephalic neurogenesis. The effect of Zic2a depletion on neurogenesis in the forebrain was examined using ISH with neuronal markers. (A, B) isl1 expression in zic2a morphants at Prim-5 shows loss of the ventrorostral cluster (VRC, arrow), and a fusion of the dorsorostral cluster (DRC, arrowhead) (35/39 embryos, 2 exp.). (C, D) sim1 expression in the preoptic area is strongly reduced (27/36 embryos, 2 exp.) at Prim-5. (E, F) otpb expression in the preoptic area is also strongly reduced (30/30 embryos, 2 exp.). (G, H) Expression of itnp, a marker of differentiated neurons at 2 dpf, is lost in zic2aMO injected embryos (28/34 embryos, 2 exp.). Embryos are shown in lateral view with anterior to the left. Insets are ventral views of the same embryos, except in panels A and B, which are anterio-ventral views. Numbers in panels C and D mark different neural clusters.

Expression Data

Genes:	isl1a otpb oxt sim1a
Fish:	AB AB + MO3-zic2a
Knockdown Reagent:	MO3-zic2a
Anatomical Terms:	preoptic area telencephalon ventral thalamus
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO3-zic2a
Knockdown Reagent:	MO3-zic2a
Observed In:	neurogenesis
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 317(1), Sanek, N.A., and Grinblat, Y., A novel role for zebrafish zic2a during forebrain development, 325-335, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.