ZFIN ID: ZDB-FIG-080502-9
Sanek et al., 2008 - A novel role for zebrafish zic2a during forebrain development. Developmental Biology   317(1):325-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Prim-5
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to 14-19 somites

Fig. 4 Zic2a regulates proliferation, but not apoptosis or differentiation of PT precursors. Embryos were injected singly or co-injected with zic2aMO and p53MO and stained out for expression of arx, an early PT marker at 15 somites (A–C), dlx2a, a late PT marker, at Prim-7 (D–F), or gfp (G, H). (A) p53 morphants show normal expression of arx (26/26 embryos, 2 exp.). (B) Embryos injected with zic2aMO alone show a strong reduction of arx expression at 15s (13/17 embryos, 2 exp.). (C) Co-injection of zic2aMO and p53MO leads to a similar reduction of arx expression (11/16 embryos, 2 exp.). (D) p53MO-injected embryos show no patterning defect at Prim-7 (29/30 embryos, 2 exp.). (E) zic2aMO-injected, or (F) zic2aMO and p53MO co-injected embryos show equivalent loss of dlx2a expression at Prim-7 (19/26 embryos, 2 exp. and 33/45 embryos, 2 exp. respectively). (G, H) Transgenic Tg(HuC:gfp) embryos express gfp in post-mitotic neurons. (G) conMO-injected transgenic embryos show no evidence of gfp-positive post-mitotic cells in the PT at 12s. (H) zic2aMO morphants do not contain prematurely differentiating cells in the PT at 12s (17/17 embryos, 3 exp.). (I) Ratios of BrdU positive cells/total cells in conMOs and zic2aMOs at 10s and 17s. 10s analysis revealed no significant difference between conMO-injected (n = 5) and zic2aMO-injected (n = 4) embryos. 17s analysis showed a significant difference (p = 0.005) between conMOs (n = 5) and zic2aMOs (n = 4). (J–M) Representative confocal sections of MO-injected embryos, showing BrdU-positive cells in yellow and BrdU-negative nuclei in green. White outlines the approximate prethalamic area determined by arx expression at the same stages. Embryos are shown in lateral view, anterior to the left. Arrows mark the PT.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arxa AB + MO3-zic2a standard conditions 14-19 somites ventral thalamus ISH
AB + MO3-zic2a + MO4-tp53 standard conditions 14-19 somites ventral thalamus ISH
AB + MO4-tp53 standard conditions 14-19 somites ventral thalamus ISH
dlx2a AB + MO3-zic2a standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
AB + MO3-zic2a + MO4-tp53 standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
AB + MO4-tp53 standard conditions Prim-5 telencephalon ISH
Prim-5 ventral thalamus ISH
EGFP knu3Tg control 10-13 somites Not Detected ventral thalamus ISH
knu3Tg + MO3-zic2a standard conditions 10-13 somites Not Detected ventral thalamus ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-BrdU IHC AB standard conditions 10-13 somites ventral thalamus
IHC 14-19 somites ventral thalamus
IHC AB + MO3-zic2a chemical treatment: 5-bromo-2'-deoxyuridine 10-13 somites ventral thalamus
IHC 14-19 somites ventral thalamus
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-zic2a chemical treatment: 5-bromo-2'-deoxyuridine 10-13 somites cell proliferation in forebrain occurrence, normal
14-19 somites cell proliferation in forebrain disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(1), Sanek, N.A., and Grinblat, Y., A novel role for zebrafish zic2a during forebrain development, 325-335, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.