ZFIN ID: ZDB-FIG-080502-6
Sanek et al., 2008 - A novel role for zebrafish zic2a during forebrain development. Developmental Biology   317(1):325-335 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 1-4 somites to 10-13 somites

Fig. 1 Zic2a is required for maintenance, but not for initiation of arx expression in the PT primordium. Embryos were stained by ISH for expression of zic2a (A–C) or arx (D–I). (A) Uninjected embryos express zic2a transiently in the early PT at 4 somites. (B, C) zic2a is not expressed in the PT at 8 and 12 somites. Prethalamus (pt) and thalamus (th) are outlined for reference. (D, E) Normal arx expression in the PT primordium of control MO injected embryos at 4, 8 and 12 somites. (G, H) arx expression in zic2a morphants. (G) arx is expressed normally at 4s (44/50 embryos, 3 exp.). (H) arx is mildly reduced at 8 somites (8/23 embryos, 2 exp.). (I) arx is drastically reduced at 12 somites (28/33 embryos, 2 exp.). (J, K) Representative parasagittal sections of conMO- and zic2aMO-injected embryos stained for arx by ISH at 10s. (L) Graph of average number of arx expressing cells in conMO (n = 3) and zic2aMO-injected (n = 3) embryos (results significant at p = 0.05). All embryos are shown with anterior to the left. Panels A, D and G are dorsal views, all others are lateral views. Arrows mark the PT primordium.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
arxa AB control 1-4 somites to 10-13 somites ventral thalamus ISH
AB + MO3-zic2a standard conditions 1-4 somites to 10-13 somites ventral thalamus ISH
zic2a AB standard conditions 1-4 somites optic primordium ISH
1-4 somites presumptive telencephalon ISH
1-4 somites ventral thalamus ISH
5-9 somites optic vesicle ISH
5-9 somites pretectum ISH
5-9 somites thalamus ISH
5-9 somites Not Detected ventral thalamus ISH
10-13 somites optic vesicle ISH
10-13 somites pretectum ISH
10-13 somites thalamus ISH
10-13 somites Not Detected ventral thalamus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-zic2a standard conditions 1-4 somites - 10-13 somites diencephalon development disrupted, abnormal
1-4 somites - 10-13 somites ventral thalamus decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(1), Sanek, N.A., and Grinblat, Y., A novel role for zebrafish zic2a during forebrain development, 325-335, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.