header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-070619-35
Qian et al., 2007 - Distinct Functions for Different scl Isoforms in Zebrafish Primitive and Definitive Hematopoiesis. PLoS Biology   5(5):e132 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15

Fig. 6 Specification of Definitive Hematopoietic Stem Cells Requires scl-β but Not scl-α (A–F) WISH of definitive hematopoietic stem cell markers c-myb (A–C) and runx1 (D–F). Expression of c-myb and runx1 in the ventral wall of DA was markedly reduced in 30-hpf scl-β morphants (C and F) but not in 30-hpf control embryos (A and D) or scl-α morphants (B and E). Insets are high-magnification (20×) views of the ventral wall of DA. The arrowheads indicate the definitive hematopoietic stem cells along the ventral wall of DA. (G–I) WISH of T cell marker rag1. rag1 expression in thymic T cells was abolished in 5-dpf scl-β morphants (I) but not in control embryos (G) and scl-α morphants (H). Arrows indicate the thymus region. In all panels, embryos are in lateral view with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myb WT control Prim-15 ventral wall of dorsal aorta ISH
WT + MO7-tal1 standard conditions Prim-15 ventral wall of dorsal aorta ISH
WT + MO8-tal1 standard conditions Prim-15 Not Detected ventral wall of dorsal aorta ISH
rag1 WT control Prim-15 T cell ISH
WT + MO7-tal1 standard conditions Prim-15 T cell ISH
WT + MO8-tal1 standard conditions Prim-15 Not Detected T cell ISH
runx1 WT control Prim-15 ventral wall of dorsal aorta ISH
WT + MO7-tal1 standard conditions Prim-15 ventral wall of dorsal aorta ISH
WT + MO8-tal1 standard conditions Prim-15 Not Detected ventral wall of dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.