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ZFIN ID: ZDB-FIG-070619-33
Qian et al., 2007 - Distinct Functions for Different scl Isoforms in Zebrafish Primitive and Definitive Hematopoiesis. PLoS Biology   5(5):e132 Full text @ PLoS Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 4 Requirement of scl-α and -β at Different Development Stages of Primitive RBC Differentiation (A–F) O-dianisidine staining of hemoglobin. At 3 dpf, expression of hemoglobin was severely reduced in scl-β morphants (C) compared to control embryos (A) and scl-α morphants (B). At 5 dpf, the o-dianisidine staining in both scl-α (E) and scl-β (F) morphants was dramatically decreased compared to that in control embryos (D). All embryos are in lateral view with anterior to the left. (G–L) May-Grunwald Giemsa staining of RBCs of different developmental stages (30 hpf to 5 dpf). Normal primitive RBCs at 30 hpf to 5 dpf can be classified into four main developmental stages (G): stage I, basophilic erythroblast; stage II, polychromatophilic erythroblast; stage III, orthochromatophilic erythroblast; and stage IV, mature erythrocyte. While RBCs from 2-dpf control embryos (H) and scl-α morphants (I) have differentiated into the polychromatophilic erythroblast stage (stage II), RBCs from scl-β morphants (J) are halted at the earlier basophilic erythroblast stage (stage I). At 4 dpf, RBCs from scl-α morphants (L) are arrested at stage II, while there is normal development of stage IV RBCs in control embryos (K). Scale bar (G–L), 10 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO7-tal1 standard conditions Day 4 nucleate erythrocyte decreased amount, abnormal
Day 4 nucleate erythrocyte immature, abnormal
Day 5 hemoglobin biosynthetic process disrupted, abnormal
WT + MO8-tal1 standard conditions Long-pec nucleate erythrocyte decreased amount, abnormal
Long-pec nucleate erythrocyte immature, abnormal
Day 5 hemoglobin biosynthetic process disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.