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ZFIN ID: ZDB-FIG-070619-32
Qian et al., 2007 - Distinct Functions for Different scl Isoforms in Zebrafish Primitive and Definitive Hematopoiesis. PLoS Biology   5(5):e132 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 20-25 somites

Fig. 3 scl-α and -β Are Functionally Redundant in the Initiation of Primitive Hematopoiesis (A–L) WISH of gata1 (A–D), βe1-globin (E–H), and pu.1 (I–L). Similar expression of gata1, βe1-globin, and pu.1 were detected in 20-hpf control embryos (A, E, and I), scl-α morphants (B, F, and J), and scl-β morphants (C, G, and K). In contrast, expression of gata1, βe1-globin, and pu.1 were significantly reduced or absent in scl-α/β co-injected morphants (D, H, and L). All embryos are in lateral view with anterior to the left. (M–T) Immunohistochemistry staining of endogenous Scl proteins in 20-hpf control embryos (M and Q) and scl-α (N and R), scl-β (O and S), and scl-α/β co-injected (P and T) morphants using Ab-Scl-N (M–P) and Ab-Scl-C (Q–T). In scl-α morphants, the Ab-Scl-N (N), but not Ab-Scl-C (R), staining in the ICM was absent, showing that scl-α MO specifically blocks the translation of scl-α. In the scl-β morphants, the Ab-Scl-C staining was selectively abolished in the APLM, where only scl-β is transcribed ([S] asterisk, inset), demonstrating that scl-β MO specifically blocks the protein expression of scl-β. In the scl-α/β co-injected morphants, neither Ab-Scl-N nor Ab-Scl-C staining was detected (P and T). Insets in (Q–T) are dorsal views of the magnified APLM marked by asterisks. Embryos are in lateral view with anterior to the left.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gata1a WT control 20-25 somites intermediate cell mass of mesoderm ISH
WT + MO7-tal1 standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
WT + MO7-tal1 + MO8-tal1 standard conditions 20-25 somites posterior lateral mesoderm ISH
WT + MO8-tal1 standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
hbbe1.1 WT control 20-25 somites intermediate cell mass of mesoderm ISH
WT + MO7-tal1 standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
WT + MO7-tal1 + MO8-tal1 standard conditions 20-25 somites posterior lateral mesoderm ISH
WT + MO8-tal1 standard conditions 20-25 somites intermediate cell mass of mesoderm ISH
spi1b WT control 20-25 somites macrophage ISH
WT + MO7-tal1 standard conditions 20-25 somites macrophage ISH
WT + MO7-tal1 + MO8-tal1 standard conditions 20-25 somites anterior lateral mesoderm ISH
WT + MO8-tal1 standard conditions 20-25 somites macrophage ISH
tal1 WT control 20-25 somites intermediate cell mass of mesoderm IHC
20-25 somites intermediate cell mass of mesoderm IHC
WT + MO7-tal1 standard conditions 20-25 somites Not Detected intermediate cell mass of mesoderm IHC
20-25 somites intermediate cell mass of mesoderm IHC
WT + MO7-tal1 + MO8-tal1 standard conditions 20-25 somites Not Detected intermediate cell mass of mesoderm IHC
20-25 somites Not Detected intermediate cell mass of mesoderm IHC
WT + MO8-tal1 standard conditions 20-25 somites intermediate cell mass of mesoderm IHC
20-25 somites posterior lateral mesoderm IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.