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ZFIN ID: ZDB-FIG-070418-12
Guner et al., 2007 - Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube. Gene expression patterns : GEP   7(5):596-605 Full text @ Gene Expr. Patterns
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: Bud to Prim-25

Fig. 2 Class II Hh regulated genes in zebrafish: nkx2.9 and nkx6.2 expression and regulation by Hh. (A and B) Lateral views of 36 hpf embryos, eyes removed, dot marks optic recess. (A) nkx2.9 is expressed in the ventral hindbrain, ventral midbrain and diencephalon in a pattern nearly identical to that of nkx2.2a (see Karlstrom et al., 2003). No nkx2.9 expression was seen outside the nervous system up to 36 hpf. Inset shows expression of nkx2.9 in the CNS at the tailbud stage (10 hpf). (B) nkx6.2 is expressed in the ventral hindbrain and ventral midbrain, but is not expressed in the forebrain. In the hindbrain, nkx6.2 is expressed dorsally, presumably in cranial motor neurons (arrows). Inset shows nkx6.2 expression in the ventral CNS at tailbud stage (10 hpf). (B′) Side view of somites 4–7 in the trunk showing nkx6.2 expression in the developing pancreas at 36 hpf. (C–H) Spinal cord cross-sections of 24 hpf embryos. (C) nkx2.9 is expressed in lateral floor plate cells, but not in the medial floor plate (*), similar to nkx2.2a (F). nkx2.9 expression is dorsally expanded in shh mRNA-injected embryos. The expression of nkx2.9 is lost in smu(smo), con(disp1), yot(gli2DR), and dtr(gli1) mutants. (D) nkx6.2 is expressed in the ventral half of the CNS. In shh mRNA-injected embryos nkx6.2 expression is expanded dorsally. nkx6.2 expression is completely absent in smu(smo) mutants. In con(disp1) mutants, nkx6.2 expression is absent dorsally but remains in the medial and lateral floor plate (arrowhead). In yot(gli2DR) and dtr(gli1) mutants, nkx6.2 expression is absent from the floor plate (arrowheads), but expression remains more dorsally. (E) nkx6.1 is expressed in lateral floor plate cells, in motor neuron precursors and in most ventral interneuron progenitors. nkx6.1 expression is dorsally expanded in shh mRNA-injected embryos and is absent in cyclopamine treated embryos. The expression of nkx6.1 is not affected in yot(gli2DR), and dtr(gli1) mutant embryos. (F) nkx2.2a is expressed in the ventral-most cells of the CNS, including the lateral floor plate but not the medial floor plate (*). (G) hlxb9 is expressed in developing motor neurons just dorsal to the floor plate. hlxb9 expression is undetectable in con(disp1) (inset) and smu(smo) mutants and highly reduced in yot(gli2DR), and dtr(gli1) mutants (data not shown). (H) olig2 is expressed in the ventral spinal cord, including in MN precursor cells (pMN). The expression of olig2 is dorsally expanded in shh mRNA-injected embryos and absent in cyclopamine treated embryos. olig2 expression appears unaffected in yot(gli2DR), and dtr(gli1) mutants. di, diencephalon; fb, forebrain; hb, hindbrain; mb, midbrain; nc, notochord; s, somite; t, telencephalon. * Marks the medial floor plate cells. Double-ended arrows in each panel show dorsal and ventral extent of gene expression. Scale bars: (A and B), 50 μm, (C–H) 15 μm in large panels, 24 μm in smaller panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mnx1 WT standard conditions Prim-5 motor neuron ISH
WT chemical treatment: Cyclopamine Prim-5 Not Detected motor neuron ISH
disp1tm15a/tm15a standard conditions Prim-5 Not Detected motor neuron ISH
smob641/b641(AB) standard conditions Prim-5 Not Detected motor neuron ISH
nkx2.2a WT standard conditions Prim-5 central nervous system ISH
Prim-5 CNS interneuron ISH
Prim-5 lateral floor plate ISH
Prim-5 Not Detected medial floor plate ISH
nkx2.9 WT standard conditions Bud neural plate ISH
Prim-5 lateral floor plate ISH
Prim-5 Not Detected medial floor plate ISH
Prim-25 Not Detected adenohypophyseal placode ISH
Prim-25 diencephalon ISH
Prim-25 hindbrain ISH
Prim-25 midbrain ISH
disp1tm15a/tm15a standard conditions Prim-5 Not Detected lateral floor plate ISH
gli1ts269/ts269 standard conditions Prim-5 Not Detected lateral floor plate ISH
gli2aty17a/ty17a standard conditions Prim-5 Not Detected lateral floor plate ISH
smob641/b641(AB) standard conditions Prim-5 Not Detected lateral floor plate ISH
nkx6.1 WT standard conditions Prim-5 CNS interneuron ISH
Prim-5 lateral floor plate ISH
Prim-5 motor neuron ISH
nkx6.2 WT standard conditions Bud neural plate ISH
Prim-5 central nervous system ISH
Prim-25 Not Detected forebrain ISH
Prim-25 hindbrain ISH
Prim-25 midbrain ISH
Prim-25 pancreatic bud ISH
disp1tm15a/tm15a standard conditions Prim-5 central nervous system ISH
gli1ts269/ts269 standard conditions Prim-5 central nervous system ISH
gli2aty17a/ty17a standard conditions Prim-5 central nervous system ISH
smob641/b641(AB) standard conditions Prim-5 Not Detected central nervous system ISH
olig2 WT standard conditions Prim-5 motor neuron ISH
Prim-5 oligodendrocyte ISH
Prim-5 spinal cord ISH
WT chemical treatment: Cyclopamine Prim-5 Not Detected motor neuron ISH
Prim-5 Not Detected oligodendrocyte ISH
Prim-5 Not Detected spinal cord ISH
gli1ts269/ts269 standard conditions Prim-5 motor neuron ISH
Prim-5 oligodendrocyte ISH
Prim-5 spinal cord ISH
gli2aty17a/ty17a standard conditions Prim-5 motor neuron ISH
Prim-5 oligodendrocyte ISH
Prim-5 spinal cord ISH
smob641/b641(AB) standard conditions Prim-5 Not Detected motor neuron ISH
Prim-5 Not Detected oligodendrocyte ISH
Prim-5 Not Detected spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Gene expression patterns : GEP for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Gene expression patterns : GEP, 7(5), Guner, B., and Karlstrom, R.O., Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube, 596-605, Copyright (2007) with permission from Elsevier. Full text @ Gene Expr. Patterns