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ZFIN ID: ZDB-FIG-060823-3
Walker et al., 2006 - Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning. Developmental Biology   295(1):194-205 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-15 to Prim-25

Fig. 6  furinA is required for early but not late edn1-dependent Dlx gene expression. Dlx expression in wild-type, furinA mutant, and edn1 mutant embryos at 30 hpf (A–L) and 36 hpf (M–U). (A–C) dlx2a, (D–F, M–O) dlx5a, (G–I, P–R) dlx6a, and (J–L, S–U) dlx3b. dlx2a expression is slightly affected in both furinA and edn1 mutant embryos, whereas expression of dlx3b, dlx5a, and dlx6a is moderately reduced in furinA mutants at 30 hpf and strongly reduced in edn1 mutants. Expression of dlx5a, dlx6a, and dlx3b recovers significantly in furinA mutants by 36 hpf. a1 and a2 label pharyngeal arch 1 and 2. Dotted lines label pharyngeal pouches 1 and 2 in (AU) and bottom of arch 1 and 2 in (O, R, and U). Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT standard conditions Prim-15 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch ISH
furinatg419/tg419 standard conditions Prim-15 pharyngeal arch ISH
dlx3b WT standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
furinatg419/tg419 standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
dlx5a WT standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
furinatg419/tg419 standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
dlx6a WT standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
furinatg419/tg419 standard conditions Prim-15 pharyngeal arch ISH
Prim-25 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 295(1), Walker, M.B., Miller, C.T., Talbot, J.C., Stock, D.W., and Kimmel, C.B., Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning, 194-205, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.