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ZFIN ID: ZDB-FIG-070918-53
Walker et al., 2006 - Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning. Developmental Biology   295(1):194-205 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 3  Both FurinA and FurinB function are required to activate Edn1 signaling. Flat mounts of Alcian-stained cartilages and bones at 5–6 dpf. (A) Wild-type, (B) furinA mutant, (C) furinA MO-injected larva, (D) furinB MO-injected larva, (E) furinB MO injected into furinA mutant, (F) low edn1 MO-injected larva, (G) edn1 mutant, and (H) dlx3b MO  + dlx5a MO-injected larva. Wild-type DV joint regions are indicated with arrows in panel A. Fusions at joint regions are indicated with asterisks in panels B–H. Scale bar: 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dlx3b + MO1-dlx5a standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
WT + MO1-edn1 standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
WT + MO1-furina standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
WT + MO1-furinb standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
edn1tf216b/tf216b standard conditions Day 5 ceratohyal cartilage aplastic, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 palatoquadrate cartilage decreased size, abnormal
furinatg419/tg419 + MO1-furinb standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
Day 5 pharyngeal arch 3 skeleton decreased size, abnormal
Day 5 pharyngeal arch 4 skeleton decreased size, abnormal
furinaunspecified/unspecified standard conditions Day 5 interhyal-epihyal joint absent, abnormal
Day 5 mandibular arch skeleton joint absent, abnormal
Day 5 pharyngeal arch 3 skeleton decreased size, abnormal
Day 5 pharyngeal arch 4 skeleton decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 295(1), Walker, M.B., Miller, C.T., Talbot, J.C., Stock, D.W., and Kimmel, C.B., Zebrafish furin mutants reveal intricacies in regulating Endothelin1 signaling in craniofacial patterning, 194-205, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.