ZFIN ID: ZDB-FIG-060706-18
Gibert et al., 2006 - Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling. Development (Cambridge, England)   133(14):2649-2659 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 6 Expression of marker genes in the mesenchyme and ectoderm of wild-type and pectoral fins. Treatment with 10 µM DEAB, starting from 16 hpf to 28 hpf (A-D), 38 hpf (I,J) and 40 hpf (E-H,K,L); and from differing starting times to 32 hpf (M-P). Anterior is towards the left. (A,B) hand2 is expressed in the medial and posterior mesenchyme of wild type (arrow), but is not expressed in the absence of RA. (C,D) shh is expressed in the posterior mesenchyme (arrow), but is not expressed in the absence of RA. (E-H) hoxd11 and hoxd12 are expressed in the posterior mesenchyme and fail to be induced in the absence of RA. (I,J) hoxc6, which is normally restricted to the anterior mesenchyme, is expanded posteriorly in DEAB-treated embryos. (K,L) dlx2a, a marker of the apical fold, is normally expressed in the absence of RA. (M-P) shh induction is lost upon early inhibition of RA signaling. Scale bars: 50 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT constant dark Prim-25 apical ectodermal ridge pectoral fin bud ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-25 apical ectodermal ridge pectoral fin bud ISH
hand2 WT constant dark Prim-5 mesoderm pectoral fin bud ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 Not Detected mesoderm pectoral fin bud ISH
hoxc6a WT constant dark Prim-25 mesenchyme pectoral fin ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-25 mesenchyme pectoral fin ISH
hoxd11a WT constant dark Prim-25 mesenchyme pectoral fin ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-25 Not Detected mesenchyme pectoral fin ISH
hoxd12a WT constant dark Prim-25 mesenchyme pectoral fin ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-25 Not Detected mesenchyme pectoral fin ISH
shha WT constant dark Prim-5 zone of polarizing activity pectoral fin bud ISH
Prim-15 zone of polarizing activity pectoral fin bud ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Prim-5 Not Detected zone of polarizing activity pectoral fin bud ISH
Prim-15 zone of polarizing activity pectoral fin bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development