FIGURE

Fig. 3

ID
ZDB-FIG-060706-15
Publication
Gibert et al., 2006 - Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling
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Fig. 3

Retinoic acid rescues pectoral fin field induction in the absence of paraxial mesoderm. Whole-mount in situ hybridizations. Anterior is towards the left, dorsal views. (A-D) Expression of tbx5 in pectoral fin buds is unchanged in ntl(B), but reduced in spt (C) and absent in ntl/spt double-mutant embryos (D). (E-M) Application of 10-8 M RA, beginning at 10 hpf, rescues tbx5 expression in ntl/spt pectoral fins (arrowheads in I,K); rescue occurs in the absence of myogenic differentiation, demonstrated by the absence of myod (J,K) and α-tropomyosin-1 (tpm1) expression (L,M) in RA-treated double mutants. Four rescue experiments, each with 40 embryos, were performed (total numbers of phenotypes: 32 spt, 35 ntl, 11 ntl/spt, 82 siblings without mutant phenotype).

Expression Data
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
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