ZFIN ID: ZDB-FIG-060706-15
Gibert et al., 2006 - Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling. Development (Cambridge, England)   133(14):2649-2659 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: Prim-5 to Prim-25

Fig. 3 Retinoic acid rescues pectoral fin field induction in the absence of paraxial mesoderm. Whole-mount in situ hybridizations. Anterior is towards the left, dorsal views. (A-D) Expression of tbx5 in pectoral fin buds is unchanged in ntl(B), but reduced in spt (C) and absent in ntl/spt double-mutant embryos (D). (E-M) Application of 10-8 M RA, beginning at 10 hpf, rescues tbx5 expression in ntl/spt pectoral fins (arrowheads in I,K); rescue occurs in the absence of myogenic differentiation, demonstrated by the absence of myod (J,K) and α-tropomyosin-1 (tpm1) expression (L,M) in RA-treated double mutants. Four rescue experiments, each with 40 embryos, were performed (total numbers of phenotypes: 32 spt, 35 ntl, 11 ntl/spt, 82 siblings without mutant phenotype).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT constant dark, chemical treatment: all-trans-retinoic acid Prim-5 somite ISH
tbx5a WT constant dark Prim-5 mesoderm pectoral fin bud ISH
WT constant dark, chemical treatment: all-trans-retinoic acid Prim-5 mesoderm pectoral fin bud ISH
tbx16b104/b104 constant dark Prim-5 mesoderm pectoral fin bud ISH
tbx16b104/b104 constant dark, chemical treatment: all-trans-retinoic acid Prim-5 mesoderm pectoral fin bud ISH
tbx16b104/b104; tbxtatc41/tc41 constant dark Prim-5 Not Detected mesoderm pectoral fin bud ISH
tbxtatc41/tc41 constant dark Prim-5 mesoderm pectoral fin bud ISH
tbxtatc41/tc41 constant dark, chemical treatment: all-trans-retinoic acid Prim-5 mesoderm pectoral fin bud ISH
tpm1 WT constant dark, chemical treatment: all-trans-retinoic acid Prim-25 somite ISH
tbx16b104/b104; tbxtatc41/tc41 constant dark, chemical treatment: all-trans-retinoic acid Prim-25 Not Detected somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development