ZFIN ID: ZDB-FIG-060706-19
Gibert et al., 2006 - Induction and prepatterning of the zebrafish pectoral fin bud requires axial retinoic acid signaling. Development (Cambridge, England)   133(14):2649-2659 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: Long-pec

Fig. 7 RA requirement in pectoral fins after 28 hpf. (A-C) Alcian Blue stains of larval pectoral fin and girdle at 6 (A,B) and 4,5 dpf (C). Treatment with 10 µM DEAB from 10-17 hpf inhibits formation of the scapulocoracoid (sco) (B), and from 28-50 hpf affects differentiation in the proximal skeletal elements of the pectoral girdle (C). (D-G) In situ hybridizations of pectoral fins at 50 hpf; differentiation of the fin mesenchyme (D,E), appendicular muscles (F) and apical fold (G) appear unchanged upon treatment with 10 µM DEAB. cl, cleithrum; ed, endoskeletal disc.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT constant dark Long-pec fin fold pectoral fin bud ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Long-pec fin fold pectoral fin bud ISH
msx3 WT constant dark Long-pec mesenchyme pectoral fin ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Long-pec mesenchyme pectoral fin ISH
myod1 WT constant dark Long-pec pectoral fin musculature ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Long-pec pectoral fin musculature ISH
tbx5a WT constant dark Long-pec mesenchyme pectoral fin ISH
WT constant dark, chemical treatment: 4-(diethylamino)benzaldehyde Long-pec mesenchyme pectoral fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development