FIGURE SUMMARY
Title

Ethanol down-regulates gastrula gene expression and cell movement, causing symptoms of foetal alcohol spectrum disorders

Authors
Alsakran, A.A., Gibson, B., Wong, H.Y., Heaton, C., Boreham, R., Minhas, R., Ball, J., Kudoh, T.
Source
Full text @ Biol. Open
Fig. 1.

Morphological defects observed in the ethanol-treated embryos at late blastula (A-E), late gastrula (F-J), 24 hpf (K-O) and 78 hpf (P-S). Scale bars: 500 µm (A-O), 2 mm (R-S).
Fig. 2.

Tg(h2a:gfp) zebrafish embryos reveal dose-dependent delay of epiboly. The Tg(h2a:gfp) embryos shows expansion of the blastoderm during gastrulation. (A-D) 6 hpf, (E-H) 7 hpf, (I-L) 8 hpf, (M-P) 8 hpf and (Q-T) 9 hpf. (U) Measurement of progress of epiboly in the control- and ethanol-treated embryos revealing dose-dependent delay of epiboly by ethanol from 0%, 1%, 2% to 3%. Scale bar: 500 µm.
Fig. 3.

Tg(gsc:gfp) zebrafish embryos reveal dose dependent delay of epiboly. The Tg(gsc:gfp) line shows expansion of the blastoderm during gastrulation. (A-D) 7 hpf, (E-H) 8 hpf, (I-L) 9 hpf, (M-P) 10 hpf. (Q) Measurement of progress of epiboly in the control- and ethanol-treated embryos revealing dose-dependent delay of epiboly by ethanol from 0%, 1%, 2% to 3%. Scale bar: 500 µm.
Fig. 4.

Timing specific gastrulation cell movement defect induced by ethanol. Ethanol (2%) was exposed to the zebrafish embryos of Tg(h2a:gfp) (A) and Tg(gsc:gfp) (B) at different stages (2-10 h, 5-10 h or 7-10 hpf). All ethanol-treated embryos at different timings showed similar cell movement defects.
Fig. 5.

Expression of endoderm and mesoderm genes are suppressed and/or altered by ethanol.In situ hybridisation staining of late gastrula embryos stained with sox17 (A-C), ntl (D-F), gsc (G-I), eve1 (J-L) and bmp4 (M-O). Scale bar: 250 µm.
Fig. 6.

Expression of ectoderm genes are suppressed and/or altered by ethanol.In situ hybridisation staining of late gastrula embryos stained with p63 (A-C), hoxb1b (D-F), sox3 (G-I), otx2 (J-L) and cyp26a1 (M-O). Scale bar: 250 µm.

EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: 90%-epiboly
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: 90%-epiboly
Fig. 7.

Expression of neural ectoderm gene sox3 is reduced or delayed by ethanol in a stage specific manner at blastula to gastrula stages. (A-C) 5 hpf, (D-F) 9 hpf, (G-I) 11 hpf. e, ectoderm; ane, anterior neural ectoderm; pne, posterior neural ectoderm. Scale bar: 250 µm.

EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: 30%-epiboly to 1-4 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: 90%-epiboly to 1-4 somites
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biol. Open
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