FIGURE

Fig. 5

ID

ZDB-FIG-260617-11

Publication

Martin et al., 2026 - Nr2f1a and Isl1 repress acquisition of epicardial identity in venous atrial cardiomyocytes

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Co-expression of cardiomyocyte markers with fgf13a:EGFP in nr2f1a;isl1 mutant hearts. (A-C‴) Optical sections of the venous pole of wild-type and nr2f1a^−/−;isl1^−/− hearts at 96 hpf stained for Myh6 (blue), myl7:DsRed2-NLS (red) and fgf13a:EGFP (green). (A-A‴) Myh6⁺/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ cells (pacemaker cardiomyocytes) in wild-type heart (white arrows). (B-B‴) Individual Myh6⁺/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ cells in nr2f1a^−/−;isl1^−/− heart (yellow arrows). (B-B‴) Myh6,sup>+/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ AV node cardiomyocytes in nr2f1a^−/−;isl1^−/− heart (blue arrows). (C-C‴) Myh6⁻/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ cells in nr2f1a^−/−;isl1^−/− heart (magenta arrows). Wild type (n=8) and nr2f1a^−/−;isl1^−/− (n=5). Scale bar: 10 μm. (D,E) Quantification of distinct Myh6⁺/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ and Myh6⁻/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ cells in wild-type and nr2f1a^−/−;isl1^−/− embryos. (D) *P=0.0032; (E) **P=0.0161 (two-tailed Welch's t-test). (F) Proportion of embryos with at least one Myh6⁻/myl7:DsRed2-NLS⁺/fgf13a:EGFP⁺ cell. *P=0.007 (Fisher's exact test).

Expression Data

Genes:	DsRed2 EGFP
Antibody:	Ab-S46
Fish:	isl1a^{ci1032/ci1032}; nr2f1a^{ci1009/ci1009}; f2Tg/f2Tg; sqet33mi59BEt/sqet33mi59BEt
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development