FIGURE

Fig. 10

ID

ZDB-FIG-260618-4

Publication

Martin et al., 2026 - Nr2f1a and Isl1 repress acquisition of epicardial identity in venous atrial cardiomyocytes

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Fig. 10

tcf21 is required to promote epicardial cell gene expression in nr2f1a;isl1 mutant atria. (A-D‴) Optical sections of immunohistochemistry for Myh6 (blue), Myh7 (red) and tcf21:NLS-EGFP (green) in control and tcf21 knockdown (KD) wild-type and nr2f1a^−/−;isl1^−/− hearts. (A-A‴) Epicardial cells (ECs) on the ventricle (white arrowheads). (B-B‴) The lack of ECs on the ventricle (yellow arrowheads). (C-C‴) tcf21:NLS-EGFP⁺/Myh6⁻ cells in myocardial wall (magenta arrowheads). (D-D‴) Gaps in Myh6 within the myocardial wall (blue arrowheads). Scale bar: 25 μm. Control: wild type (n=9) and nr2f1a^−/−;isl1^−/− (n=6); tcf21 KD: wild type (n=9) and nr2f1a^−/−;isl1^−/− (n=5). (E) Quantification tcf21:NLS-EGFP⁺ cells within the control and tcf21 KD nr2f1a^−/−;isl1^−/− hearts. *P=0.0106 (two-tailed Welch's t-test). (F) Quantification of gaps in Myh6⁺ myocardial wall of control and tcf21 KD nr2f1a^−/−;isl1^−/− hearts. *P=0.0161 (two-tailed Welch's t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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