Fig. 7

pmpcb deficiency leads to activation of the ER stress response. A Reactive oxygen species (ROS) levels in the WT siblings, NAC exposed WT siblings, pmpcb−/− larvae and the NAC exposed pmpcb−/− larvae at 72 hpf. B qRT-PCR assays for the ER stress-related genes in the WT siblings, PBA exposed WT siblings, pmpcb−/− larvae and the PBA exposed pmpcb−/− larvae at 72 hpf. C Transcriptional expression of bip at 24 and 72 hpf in WT siblings and pmpcb−/− embryos and larvae (C, left), and the calculation of the relative expression levels of bip (C, right). D, E Transcriptional expression of bip at 24 hpf in the WT siblings, PBA exposed WT siblings, pmpcb−/− embryos and the PBA exposed pmpcb−/− embryos (D), and the calculation of the relative expression level of bip (E). F WB analysis of Calnexin, Atf4, Eif2α and the phosphorylation of Eif2α at 72 hpf in in the WT siblings, PBA exposed WT siblings, pmpcb−/− larvae and the PBA exposed pmpcb−/− larvae (F, left), and the calculation of the relatively translational levels of Calnexin, Atf4, Eif2α and the phosphorylation of Eif2α (F, right). G, H Transcriptional expression of gfap and vimentin at 24 (G, left) and 72 hpf (H, left) in the WT siblings, PBA exposed WT siblings, pmpcb−/− mutants and the PBA exposed pmpcb.−/− mutants, and the calculation of the relative expression levels of gfap and vimentin (G, right, H, right). PBA, ER stress scavenger 4-phenylbutyric acid. A, C, D, G, H, lateral view, anterior to the left, and dorsal to the up. Each experiment was repeated at least three times, and a representative result is shown. Data are analyzed using GraphPad Prism 9.5 for t-test, and are presented as mean ± SD. ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant. Scale bar, 200 μm (A, C, D, G, H)