Fig. 2

Pmpcb deficiency impairs the generation of different nervous cells. A Expression of sox2 at 24, 48 and 72 hpf in WT siblings, pmpcb± and pmpcb−/− embryos and larvae (A, left), and the calculation of the relative expression levels of sox2 (A, right). B, C Transcriptional expression of gfap at 24, 48, 72 and 96 hpf in WT siblings, pmpcb± and pmpcb−/− embryos and larvae (B), and the calculation of the relatively transcriptional levels of gfap (C). D, E Transcriptional expression of mbp at 72 and 96 hpf in zebrafish larvae (D), and the calculation of the relative expression levels (E). F WB analysis of Sox2 and Gfap at 72 hpf in WT siblings and pmpcb−/− larvae (F, left), and the calculation of the relative expression levels of Sox2 and Gfap (F, right). G-I Immunofluorescence was used to detect the expression of Sox2 in sectioned brain in WT siblings and pmpcb−/− larvae at 72 hpf (G), and the number of Sox2+ cells (I, H left) and the relative Sox2.+ fluorescence (H, right) were calculated. A, B, D, lateral view, anterior to the left, and dorsal to the up. Red arrows indicate the positive signals in CNS. Each experiment was repeated at least three times, and a representative result is shown. Data are analyzed using GraphPad Prism 9.5 for t-test, and are presented as mean ± SD. ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant. Scale bar, 200 μm (A, B, D), 50 μm (G), 25 μm (G, Enlarged)