Fig. 6

WNT agonist BIO and ATP precursor creatine effectively recover the neurodevelopmental phenotype. A, B Transcriptional expression of the mature glial cell markers gfap and vimentin in the WT siblings, BIO exposed WT siblings, pmpcb−/− mutants and the BIO exposed pmpcb−/− mutants at 24 (A, left) and 72 hpf (B, left). A, B, right, Calculation of the relatively transcriptional levels of gfap and vimentin in different samples. C Transcriptional expression of gfap, vimentin and axin2 in the WT siblings, CRE exposed WT siblings, pmpcb−/− embryos and the CRE exposed pmpcb−/− embryos at 24 hpf (C, left). C, right, Calculation of gfap, vimentin and axin2 expression levels in different samples. CRE, stimulating cellular ATP production. D Transcriptional expression of gfap and vimentin in the WT siblings, CRE exposed WT siblings, pmpcb−/− larvae and the CRE exposed pmpcb.−/− larvae at 72 hpf (D, left). D, right, Calculation of gfap and vimentin expression levels in different samples. A-D, lateral view, anterior to the left, and dorsal to the up. Each experiment was repeated at least three times, and a representative result is shown. Data are analyzed using GraphPad Prism 9.5 for t-test, and are presented as mean ± SD. ***P < 0.001, **P < 0.01, *P < 0.05, ns, not significant. Scale bar, 200 μm (A-D)