Fig. 5

Pmpcb deficiency leads to the downregulated WNT signaling. A Expression of axin2 in WT siblings and pmpcb−/− embryos and larvae at 24 and 72 hpf respectively (A, left), and the calculation of the relatively transcriptional levels of axin2 (A, right). B WB analysis of β-Catenin and β-Catenin-pS552 in WT siblings and pmpcb−/− larvae at 72 hpf (B, left), and the calculation of the relatively translational levels of β-Catenin and β-Catenin-pS552 (B, right). C, D Immunofluorescence was used to detect the expression of β-Catenin and Gfap in sectioned brain in WT siblings and pmpcb−/− larvae at 72 hpf (C), and the number of β-Catenin+Gfap+ cells (D, left) and the relative β-Catenin+ and Gfap+ fluorescence (D, right) were calculated respectively. E–G The relatively fluorescence intensity of β-Catenin in each individual Gfap+ cell in brain and eye of the WT siblings and pmpcb.−/− larvae at 72 hpf. A, lateral view, anterior to the left, and dorsal to the up. Each experiment was repeated at least three times, and a representative result is shown. Data are analyzed using GraphPad Prism 9.5 for t-test, and are presented as mean ± SD. ***P < 0.001. Scale bar, 200 μm (A), 50 μm (C), 10 μm (E)