FIGURE

Fig. 5

ID

ZDB-FIG-250925-50

Publication

Epting et al., 2025 - Tulp3 deficiency results in ciliopathy phenotypes during zebrafish embryogenesis

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Fig. 5

Tulp3 deficiency results in the upregulation of cilia-dependent signalling pathway components in zebrafish. (A) qPCR analysis reveals unaltered expression of Hh signalling components (gli1, ptc1) and Wnt signalling component axin2 while Wnt signalling components wnt8a and lef1 were upregulated upon TB/SB1-MO tulp3 mediated knockdown compared to the respective control at 1dpf. (B) qPCR analysis reveals unaltered expression of Hh signalling components (gli1, ptc1) and Wnt signalling component axin2 while Wnt signalling components wnt8a and lef1 or Jak/Stat signalling components jak1 and stat1b were upregulated in MZtulp3 embryos compared to the respective control at 1dpf. (C) WISH analyses indicate unaltered expression of Hh signalling component ptc1 and Wnt signalling component axin2 while Wnt signalling components wnt8a and lef1 were upregulated in MZtulp3 embryos compared to the respective control at 1dpf. (D) Heatmap of Wnt signalling pathway associated gene ontology (GO)-terms (biological process, BP) of MZtulp3 embryos compared to control embryos at 2dpf. Columns represent up- or downregulated processes in MZtulp3 mutant embryos compared to controls.

Expression Data

Genes:	axin2 gli1 jak1 lef1 ptch1 stat1b wnt8a
Fish:	WT tulp3^uf3/uf3 WT + MO2-tulp3 WT + MO3-tulp3
Knockdown Reagents:	MO2-tulp3 MO3-tulp3
Anatomical Term:	whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tulp3^uf3/uf3 WT + MO2-tulp3 WT + MO3-tulp3
Knockdown Reagents:	MO2-tulp3 MO3-tulp3
Observed In:	positive regulation of receptor signaling pathway via JAK-STAT smoothened signaling pathway whole organism Wnt signaling pathway
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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