FIGURE

Figure S1.

ID

ZDB-FIG-250705-18

Publication

Abgueguen et al., 2025 - Sephin1 reduces TDP-43 cytoplasmic mislocalization and improves motor neuron survival in ALS models

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Figure S1.

Glutamate intoxication decreases cell viability, neurite network integrity, and increases extranuclear TDP-43 in primary motor neurons from WT and SOD1^G93A rats.

(A) Representative picture of immunoblot obtained by WES automated WB apparatus for caspase 3 and cleaved caspase 3 proteins in primary WT rat motor neurons intoxicated with glutamate and treated with DMSO or Sephin1 50 nM. (B) Cell viability in control and glutamate intoxicated primary WT rat motor neurons. (C) Total neurite length in control and glutamate intoxicated primary WT rat motor neurons. (D) Percentage of extranuclear TDP-43 in control and glutamate intoxicated primary WT rat motor neurons. (E) Cell viability in control and glutamate intoxicated primary SOD1^G93A rat motor neurons. (F) Total neurite length in control and glutamate intoxicated primary SOD1^G93A rat motor neurons. (G) Percentage of extranuclear TDP-43 in control and glutamate intoxicated primary SOD1^G93A rat motor neurons. (H) Mitochondrial ROS level in control and glutamate intoxicated primary SOD1^G93A rat motor neurons. *P < 0.05, **P < 0.01 Mann-Whitney test, mean ± SEM n = 4–6 replicates per condition.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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