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Impact of TDP-43G348C protein expression in zebrafish embryos on survival and locomotor function. (A) Immunoblot of TDP-43 in zebrafish embryos. The C-terminal TDP-43 antibody detects a band ($) at ∼75 kD in the zebrafish embryos expressing TDP-43G348C protein only. (B) Percentage of survival of zebrafish embryos treated with vehicle or Sephin1 10 μM (n = 8–12 replicates per condition). (C) Motor dysfunction in zebrafish embryos treated with vehicle or Sephin1 10 μM (n = 8–12 replicates per condition). (D) Swin distance measured in zebrafish embryos treated with vehicle or Sephin1 10 μM after the touch of the tail (n = 4–50 zebrafish per condition). (E) Swim duration measured in zebrafish embryos treated with vehicle or Sephin1 10 μM after the touch of the tail (n = 4–50 zebrafish per condition). (F) Swim velocity measured in zebrafish embryos treated with vehicle or Sephin1 10 μM after the touch of the tail (n = 4–50 zebrafish per condition). Ntg: WT zebrafish; Ntg-HS: WT zebrafish with two heat shock; mTDP43: zebrafish harboring the mTDP43 transgene without expressing the TDP43G348C protein; mTDP43-HS: zebrafish expressing TDP43G348C protein. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 Kruskal-Wallis test followed by Dunn’s multiple comparison test; mean ± SEM. Source data are available for this figure.
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