Fig. Ext 6

a, Scheme for human fetal tissue dissection, multiome, and data analysis, with UMAPs of merged datasets for two outer-ear replicates (teal, green), epiglottis (purple), and nose (yellow) cells. b, Feature plot shows COL2A1 mRNA expression, with arrows pointing to the two distinct COL2A1-high cartilage clusters. c, UMAPs by tissue identity. d, Cluster identities for the merged snRNAseq datasets. e, Bar chart plotting proportion of each cell type found within the different libraries. f, Violin plots showing COL2A1 and ELN mRNA expression in cells from the four cartilage clusters separated by tissue identity. g, Venn diagrams illustrating the proportion of ATAC peaks differentially accessible in outer-ear versus nose cartilage that are also differentially accessible in epiglottis versus nose cartilage. h, Coverage plots showing accessibility of human outer-ear elastic cartilage enhancers with cross-species gill-specific activity and nose cartilage enhancers with cross-species hyaline cartilage activity across all four human snATACseq libraries. Grey bars designate the peaks tested in transgenic assays.