Fig. 8

The stacked column chart illustrates the percentage of developing embryos with isolated neural tube defect (INTD) at 120 hpf in groups exposed to decitabine alone and in the presence of myo-inositol (n=20). The phenotypic outcomes with isolated neural tube defects (INTDs) noted in groups exposed decitabine 1 mM was lordosis (5%) and the remaining 95% suffered from multiple neural tube defects. In group exposed to dectabine 1 mM and myo-inositol 50 µM, the phenotypic outcomes with isolated neural tube defects were lordosis (5%), scoliosis (5%) and neural tube curvature (5%). 50% of developing embryos suffered from multiple neural tube defects and 35% was observed normal. In group exposed to dectabine 1 mM and myo-inositol 100 µM, the phenotypic outcomes with isolated neural tube defects were lordosis (5%), wavy neural tube (5%), curly up-tail curvature (5%). 55% of developing embryos suffered from multiple neural tube defects and 30% of developing embryos were observed normal