Fig. 5

Representative images of developing embryos at 120 hpf in groups exposed to DCT alone and DCT with MI. At 120 hpf, all developing embryos in the DCT group showed abnormalities in the developing neural tube at multiple sites. The deformities specified were kyphosis (K), scoliosis (S), wavy neural tube (WNT), pericardial edema (PE), neural tube curvature (NTC), tail curvature (TC), craniofacial malformation (CFM), spinal split (SS) and curly-uptail curvature (CUC).Embryos in the DCT and MI 50 µM and 100 µM groups hadboth normal neural tube development and neural tube deformitiesat multiple sites.The deformities specified in embryos exposed to DCT and MI 50 µM were pericardial edema (PE), lordosis (L), neural tube curvature (NTC), kyphosis (K), craniofacial malformation (CFM), wavy neural tube (WNT), tail curvature (TC), curly-up tail curvature (CUC) and scoliosis (S). The deformities specified in embryos exposed to DCT and MI 100 µM were curly-up tail curvature (CUC), wavy neural tube (WNT), pericardial edema (PE), craniofacial malformation (CFM), tail curvature (TC), scoliosis (S), neural tube curvature (NTC), wavy neural tube (WNT) and lordosis (L). The deformities in developing neural tube are indicated by arrow marks