FIGURE

Video 3.

ID
ZDB-FIG-250415-84
Publication
Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva
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Video 3.

Imaris 3D rendering and colocalization of RNAscope spots and hematopoietic stem and progenitor cells (HSPCs) in the CHT region.

3D reconstitution generated from a spinning disk confocal z-stack obtained from a 5 dpf Tg(runx1+23:eGFP) larva. eGFP (green) under the control of the runx1+23 enhancer is expressed in HSPCs. RNAscope signals (magenta) colocalize in the majority with HSPCs (see also Figure 6 for maximum z-projections and single z-sections and Figure 7 for 3D reconstitution and cell segmentation steps).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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