FIGURE

Figure 7.

ID

ZDB-FIG-250415-83

Publication

Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

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Figure 7.

Cell and RNAscope segmentation workflow with Imaris.

All data shown are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope, spots in magenta) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer (the same 5 dpf larva and images as in Figure 6). (A) File conversion with Imaris File Converter. (B) Imaris 3D visualization in surpass mode. (C) Step-by-step workflow of cell segmentation, as detailed in Data Analysis, Section B. 3D cells and RNAscope signal segmentation with Imaris – Cell Biologist package.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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