FIGURE

Video 2.

ID

ZDB-FIG-250415-81

Publication

Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

Other Figures

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Video 2.

Imaris 3D reconstitution of RNAscope in situ cmyb expression analysis in the pronephros region at 5 dpf.

3D rendering (reconstituted with the Imaris software) obtained from a spinning disk confocal image (z-stack) obtained from a 5 dpf Tg(kdrl:eGFP) larva. The eGFP (green) under the control of the kdrl vascular promoter is expressed in endothelial cells. Z-stack shows the pronephros region. The cmyb RNAscope signals (magenta) are localized in hematopoietic stem and progenitor cells (HSPCs) accumulated in the peri-glomerular region, surrounded by vessels. Unique z-sections and maximum z-projection are also shown in Figure 5, and the complete z-stack is shown in Video 1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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