FIGURE

Figure 1.

ID

ZDB-FIG-250415-75

Publication

Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

Other Figures

All Figure Page

Back to All Figure Page

Figure 1.

Summary of the main steps of the RNAscope procedure.

AMP: signal AMPlifier (1, 2, or 3); HRP: HorseRadish peroxidase; HRP-C: HRP channel (1, 2, or 3); TSA: tyramide signal amplification; ZZ: pair of oligonucleotide probes [with target-specific binding sites covering regions of approximately 50 bases and mRNA target regions covering 300–1,000 nucleotides (with a pool of 6–20 ZZ pairs)]. OR + grey arrow indicates, on Day 2, the repeat of steps involved in additional fluorophore coupling using HRP activity in case of probe multiplexing using other channels (two times if two probes, three times if three probes) or interruption of the procedure until mounting of embryos/larvae on Day 3.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Bio Protoc